Special Section on Optical Imaging, Sensing, and Light Interactions in Cells and Tissues

Transcutaneous delivery of micro- and nanoparticles with laser microporation

[+] Author Affiliations
Elina A. Genina, Alexey N. Bashkatov, Leonid E. Dolotov, Vyacheslav I. Kochubey

Saratov State University, Research-Educational Institute of Optics and Biophotonics, 83 Astrakhanskaya Street, Saratov 410012, Russia

Galina N. Maslyakova

Saratov State Medical University, Department of Pathological Anatomy, 112 Bolshaya Kazachia, Saratov 410012, Russia

Ilya V. Yaroslavsky, Gregory B. Altshuler

Cynosure Inc., 15 Network Drive, Burlington, Massachusetts 01803

Valery V. Tuchin

Saratov State University, Research-Educational Institute of Optics and Biophotonics, 83 Astrakhanskaya Street, Saratov 410012, Russia

Institute of Precise Mechanics and Control of RAS, Laboratory of Laser Diagnostics of Technical and Living Systems, 24 Rabochaya Street, Saratov 410028, Russia

University of Oulu, Optoelectronics and Measurement Techniques Laboratory, P.O. Box 4500, Oulu FIN-90014, Finland

J. Biomed. Opt. 18(11), 111406 (Jul 15, 2013). doi:10.1117/1.JBO.18.11.111406
History: Received March 6, 2013; Revised June 13, 2013; Accepted June 17, 2013
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Abstract.  Fractional laser ablation is one of the relatively safe and minimally invasive methods used to administer micro- and nanoparticles into the skin at sufficiently large depth. In this article, we present the results of delivery of TiO2 nanoparticles and Al2O3 microparticles into skin. Fractional laser microablation of skin was provided by a system based on a pulsed Er:YAG laser with the following parameters: the wavelength 2940 nm, the pulse energy 3.0 J, and the pulse duration 20 ms. Ex vivo and in vivo human skin was used in the study. The suspensions of titanium dioxide and alumina powder in polyethylene glycol with particle size of about 100 nm and 27 μm, respectively, were used. In the ex vivo experiments, reflectance spectra of skin samples with administered particles were measured and histological sections of the samples were made. In the in vivo experiment, reflectance spectroscopy, optical coherence tomography, and clinical photography were used to monitor the skin status during one month after suspension administering. It is shown that particles can be delivered into dermis up to the depth 230 μm and distributed uniformly in the tissue. Spectral measurements confirm that the particles stay in the dermis longer than 1 month.

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© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Elina A. Genina ; Alexey N. Bashkatov ; Leonid E. Dolotov ; Galina N. Maslyakova ; Vyacheslav I. Kochubey, et al.
"Transcutaneous delivery of micro- and nanoparticles with laser microporation", J. Biomed. Opt. 18(11), 111406 (Jul 15, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.11.111406


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