Restricting the analysis to the mice showing clear seizure activity, we aimed to investigate whether seizure duration impacted network changes. To calculate the changes of bilateral correlation, we subtracted the bilateral correlation during control sessions, , from the bilateral correlation during the post 4-AP injection sessions, , and plotted this change versus the average seizure duration of the 4-AP sessions, as illustrated in Fig. 4. Overall correlations between seizure duration and changes in bilateral correlations were moderate. For the somatosensory cortex, where the toxin was injected, there was a positive correlation between the duration of the seizure and the changes in bilateral correlation. This same positive correlation was observed from the data extracted from the frontal cortex seeds. For other cortical regions, i.e., motor, cingulate, retrosplenial, and visual, a reverse effect was observed: the changes were negatively correlated with the seizure duration. For CBF contrast, the correlation showed to be significant in the somatosensory () and retrosplenial () regions. HbR contrast had a significant correlation for retrosplenial seeds (), while displayed a moderate (not significant) correlation for the motor seeds (). The average length of seizures per subject was . All recording sessions were 863 s long. Table 1 provides the duration of seizure-like activity for every recording session, the percentage of the recording session that belongs to seizure-like activity, and the average duration of seizures per subject.