Research Papers: General

High-throughput fabrication of gray-level biomicrostructures via temporal focusing excitation and laser pulse control

[+] Author Affiliations
Yi-Cheng Li, Li-Chung Cheng, Chia-Yuan Chang

National Cheng Kung University, Department of Photonics, Tainan 701, Taiwan

Chun-Yu Lin, Nan-Shan Chang

National Cheng Kung University, Institute of Molecular Medicine, Tainan 701, Taiwan

Paul J. Campagnola

University of Wisconsin–Madison, Department of Biomedical Engineering, Madison, Wisconsin 53706

Chen Yuan Dong

National Taiwan University, Department of Physics, Taipei 106, Taiwan

Shean-Jen Chen

National Cheng Kung University, Department of Engineering Science, Tainan 701, Taiwan

National Cheng Kung University, Advanced Optoelectronic Technology Center, Tainan 701, Taiwan

National Cheng Kung University, Center for Micro/Nano Science and Technology, Tainan 701, Taiwan

J. Biomed. Opt. 18(7), 075004 (Jul 25, 2013). doi:10.1117/1.JBO.18.7.075004
History: Received April 2, 2013; Revised June 6, 2013; Accepted June 28, 2013
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Abstract.  A developed temporal focusing-based multiphoton excitation system with additional patterned excitation and local laser control can provide high-throughput fabrication of three-dimensional gray-level biomicrostructures via two-photon cross-linking with rose bengal (RB) as the photoactivator. Multiple bovine serum albumin (BSA) structures of different concentrations were simultaneously achieved by selecting different pulse numbers in the designated regions with an appropriate femtosecond laser power within a few seconds. Intensity of the RB two-photon excited fluorescence (TPEF) is correlated to the concentration of the fabricated cross-linked BSA microstructure. Hence, the fabricated BSA microstructure can be monitored online by utilizing the RB TPEF as contrast agent. It suggests that this approach with its unique capability of high-speed, gray-level, and online-inspection fabrication meets the requirements of the biomedical researches involved in extracellular matrix.

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© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Yi-Cheng Li ; Li-Chung Cheng ; Chia-Yuan Chang ; Chun-Yu Lin ; Nan-Shan Chang, et al.
"High-throughput fabrication of gray-level biomicrostructures via temporal focusing excitation and laser pulse control", J. Biomed. Opt. 18(7), 075004 (Jul 25, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.7.075004


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