Special Section on Fluorescence Molecular Imaging Honoring Prof. Roger Tsien, a Pioneer in Biomedical Optics

LipImage™ 815: novel dye-loaded lipid nanoparticles for long-term and sensitive in vivo near-infrared fluorescence imaging

[+] Author Affiliations
Aurélie Jacquart, Emilie Rustique, Frédérique Mittler, Fabrice P. Navarro, Jérôme Boutet, Isabelle Texier

CEA Grenoble, LETI-DTBS, MINATEC campus, F38054 Grenoble, France

Michelle Kéramidas, Julien Vollaire, Jean-Luc Coll

INSERM-UJF U823, Institut Albert Bonniot, 38706 Grenoble, France

Raphaël Boisgard, Géraldine Pottier

SHFJ, CEA Orsay, 4 place Général Leclerc, 91401 Orsay Cedex, France

J. Biomed. Opt. 18(10), 101311 (Jul 30, 2013). doi:10.1117/1.JBO.18.10.101311
History: Received February 28, 2013; Revised June 7, 2013; Accepted June 27, 2013
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Abstract.  A new contrast agent, LipImage™ 815, has been designed and compared to previously described indocyanine green (ICG)-loaded lipid nanoparticles (ICG-lipidots®). Both contrast agents display similar size (50-nm diameter), zeta potential, high IC50 in cellular studies, near-infrared absorption and emission wavelengths in the “imaging window,” long-term shelf colloidal and optical stabilities with high brightness (>106Lmol1cm1) in ready-to-use storage conditions in aqueous buffer (4°C in dark), therefore being promising fluorescence contrast agents for in vivo imaging. However, while ICG-lipidots® display a relatively short plasma lifetime, LipImage™ 815 circulates in blood for longer times, allowing the efficient uptake of fluorescence signal in human prostate cancer cells implanted in mice. Prolonged tumor labeling is observed for more than 21 days.

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© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Aurélie Jacquart ; Michelle Kéramidas ; Julien Vollaire ; Raphaël Boisgard ; Géraldine Pottier, et al.
"LipImage™ 815: novel dye-loaded lipid nanoparticles for long-term and sensitive in vivo near-infrared fluorescence imaging", J. Biomed. Opt. 18(10), 101311 (Jul 30, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.10.101311


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