Research Papers: Sensing

Native fluorescence spectroscopy reveals spectral differences among prostate cancer cell lines with different risk levels

[+] Author Affiliations
Yang Pu

City College of the City University of New York, Institute for Ultrafast Spectroscopy and Lasers, Department of Physics, New York, New York 10031

Washington University School of Medicine, 4525 Scott Avenue, St. Louis, Missouri 63110

Jianpeng Xue

China Pharmaceutical University, Department of Biomedical Engineering, Nanjing 210009, China

Washington University School of Medicine, 4525 Scott Avenue, St. Louis, Missouri 63110

Wubao Wang, Robert R. Alfano

City College of the City University of New York, Institute for Ultrafast Spectroscopy and Lasers, Department of Physics, New York, New York 10031

Baogang Xu, Rui Tang, Samuel Achilefu

Washington University School of Medicine, 4525 Scott Avenue, St. Louis, Missouri 63110

Yueqing Gu

China Pharmaceutical University, Department of Biomedical Engineering, Nanjing 210009, China

Ellen Ackerstaff

Memorial Sloan Kettering Cancer Center, Department of Medical Physics, New York

Jason A. Koutcher

Memorial Sloan Kettering Cancer Center, Department of Medical Physics, New York

Memorial Sloan Kettering Cancer Center, Department of Medicine, New York

Memorial Sloan Kettering Cancer Center, Department of Radiology, New York

Cornell University, Weill Cornell Medical College, New York

J. Biomed. Opt. 18(8), 087002 (Aug 02, 2013). doi:10.1117/1.JBO.18.8.087002
History: Received March 27, 2013; Revised June 27, 2013; Accepted July 2, 2013
Text Size: A A A

Abstract.  The spectral changes of native fluorophores among normal fibroblasts and cancer cell lines of different metastatic ability are investigated by fluorescence spectroscopy. The normal (fibroblast), moderately metastatic (DU-145), and advanced metastatic (PC-3) cell lines were each selectively excited at 300 nm, and their fluorescence emission spectra are analyzed using principal component analysis to explore the differences of the relative contents of tryptophan and reduced nicotinamide adenine dinucleotide in these cell lines. The results show that the tryptophan emission featured predominantly in the fluorescence spectra of the advanced metastatic cancer cells in comparison with the moderately metastatic cancer and normal cells.

Figures in this Article
© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Yang Pu ; Jianpeng Xue ; Wubao Wang ; Baogang Xu ; Yueqing Gu, et al.
"Native fluorescence spectroscopy reveals spectral differences among prostate cancer cell lines with different risk levels", J. Biomed. Opt. 18(8), 087002 (Aug 02, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.8.087002


Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement


  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.