This book has several unique features that work to fulfill the author’s aim. In a short book that covers the entire field of single-molecule cellular biophysics, an innovative approach is required. Each chapter begins with a quotation that introduces a unique viewpoint about the material contained in the chapter. Then the content of the chapter is presented in a paragraph labeled “General Idea.” The text is supplemented with small but high-resolution black and white figures. These figures are clearly labeled, and the figure legends are sufficiently detailed to be independent of the text. There are boxes in each chapter that are labeled “Physics-Extra” or “Bio-Extra” that contain details aimed at readers with backgrounds in either physics or the life sciences. Throughout the text, “Key Point” boxes appear to highlight the salient facts for the reader. Each chapter ends with several useful features. First there is a part called “The Gist,” which consists of bulleted points of major importance, and a “Take-Home Message” to concisely summarize the chapter. The references are separated into two sections, General and Advanced, which is helpful for the reader. Second, there are two sets of questions, one for the life scientists and one for the physical scientists. And for those readers who are uncertain to which group they belong, there are additional questions in the section “for those who have not made up their mind.” I found these features and all of the end-of-chapter questions to be provocative and useful to amplify my understanding of the chapters’ contents. An index is provided.