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Special Section on Fluorescence Molecular Imaging Honoring Prof. Roger Tsien, a Pioneer in Biomedical Optics

Multispectral imaging in the extended near-infrared window based on endogenous chromophores

[+] Author Affiliations
Qian Cao, Natalia G. Zhegalova, Steven T. Wang, Walter J. Akers, Mikhail Y. Berezin

Washington University School of Medicine, Department of Radiology, St. Louis, Missouri 63110

J. Biomed. Opt. 18(10), 101318 (Aug 12, 2013). doi:10.1117/1.JBO.18.10.101318
History: Received March 2, 2013; Revised May 21, 2013; Accepted July 8, 2013
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Abstract.  To minimize the problem with scattering in deep tissues while increasing the penetration depth, we explored the feasibility of imaging in the relatively unexplored extended near infrared (exNIR) spectral region at 900 to 1400 nm with endogenous chromophores. This region, also known as the second NIR window, is weakly dominated by absorption from water and lipids and is free from other endogenous chromophores with virtually no autofluorescence. To demonstrate the applicability of the exNIR for bioimaging, we analyzed the optical properties of individual components and biological tissues using an InGaAs spectrophotometer and a multispectral InGaAs scanning imager featuring transmission geometry. Based on the differences in spectral properties of tissues, we utilized ratiometric approaches to extract spectral characteristics from the acquired three-dimensional “datacube”. The obtained images of an exNIR transmission through a mouse head revealed sufficient details consistent with anatomical structures.

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© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Qian Cao ; Natalia G. Zhegalova ; Steven T. Wang ; Walter J. Akers and Mikhail Y. Berezin
"Multispectral imaging in the extended near-infrared window based on endogenous chromophores", J. Biomed. Opt. 18(10), 101318 (Aug 12, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.10.101318


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