Research Papers: Imaging

Blind unmixing of spectrally resolved lifetime images

[+] Author Affiliations
Farzad Fereidouni, Gerhard A. Blab, Hans C. Gerritsen

Utrecht University, Debye Institute, Department of Molecular Biophysics, Utrecht 3584CC, The Netherlands

J. Biomed. Opt. 18(8), 086006 (Aug 12, 2013). doi:10.1117/1.JBO.18.8.086006
History: Received April 1, 2013; Revised June 11, 2013; Accepted June 20, 2013
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Abstract.  A method, is presented for blind unmixing spectrally resolved fluorescence lifetime images. The method is based on the combined analysis of spectral and lifetime phasors and allows unmixing of up to three components without any prior knowledge. Fractional intensities, spectra and decay curves of the individual components can be extracted with this new technique. The reliability and sensitivity are investigated and the possibility of extending the method to unmix more components is discussed. The method is evaluated on mixtures of fluorescent dyes and labeled cells.

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© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Farzad Fereidouni ; Gerhard A. Blab and Hans C. Gerritsen
"Blind unmixing of spectrally resolved lifetime images", J. Biomed. Opt. 18(8), 086006 (Aug 12, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.8.086006


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