Research Papers: Imaging

High-contrast multilayer imaging of biological organisms through dark-field digital refocusing

[+] Author Affiliations
Ahmad Faridian, Giancarlo Pedrini, Wolfgang Osten

Universität Stuttgart, Institut für Technische Optik, Pfaffenwaldring 9, D-70569 Stuttgart, Germany

J. Biomed. Opt. 18(8), 086009 (Aug 12, 2013). doi:10.1117/1.JBO.18.8.086009
History: Received April 16, 2013; Revised July 2, 2013; Accepted July 15, 2013
Text Size: A A A

Abstract.  We have developed an imaging system to extract high contrast images from different layers of biological organisms. Utilizing a digital holographic approach, the system works without scanning through layers of the specimen. In dark-field illumination, scattered light has the main contribution in image formation, but in the case of coherent illumination, this creates a strong speckle noise that reduces the image quality. To remove this restriction, the specimen has been illuminated with various speckle-fields and a hologram has been recorded for each speckle-field. Each hologram has been analyzed separately and the corresponding intensity image has been reconstructed. The final image has been derived by averaging over the reconstructed images. A correlation approach has been utilized to determine the number of speckle-fields required to achieve a desired contrast and image quality. The reconstructed intensity images in different object layers are shown for different sea urchin larvae. Two multimedia files are attached to illustrate the process of digital focusing.

Figures in this Article
© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Ahmad Faridian ; Giancarlo Pedrini and Wolfgang Osten
"High-contrast multilayer imaging of biological organisms through dark-field digital refocusing", J. Biomed. Opt. 18(8), 086009 (Aug 12, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.8.086009


Tables

Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.