Micro-fabricated cell array devices are in high demand owing to their importance in cell-to-cell interaction, drug testing, and neuronal signaling study.44–48 In this study, HeLa cells in specific micro-domains were cultured to demonstrate the feasibility of a fabricated microculture platform in cell patterning. Due to the exposed glass surface surrounded by the nonadhesive areas, cells grew in geometrical microtraps. An array of single individual cells is beneficial in understanding the mechanism and function of each cell in detail. A notable example is the study of nerve cell interaction and time-dependent behavior of a cell during the division cycle.49–51 Additionally, this work demonstrated the possibility of cell-to-cell connections through the guided patterns, which can potentially be used for a quantitative neuronal signaling study.47 For dynamic alteration of cell adhesiveness, we had to engrave patterns in situ under culture medium. Keeping this in mind, this work evaluated the size of the engraved areas in response to applied laser power under air and water environments. The smaller width of the patterns observed while engraving under water conditions was most likely owing to greater heat dissipation in water. However, engraving in air spreads the heat to a larger area, creating larger domains for the same applied laser power. This work also evaluates the effect of polymer layer on laser ablation. The polymer layer likely increases the binding of gold particles over the glass surface, acting as net-like adhesive. Thus, the ablated domain was larger when the gold coating was not over-layered with MPC polymer. Additionally, when the thickness of the gold coating increases, the absorption of photons increases as well, and this causes higher domain size than in the thinner gold layer. Figure 7 illustrates the effect of laser irradiation on the nearby cells. According to our results, 20% of the cells disappeared when the laser was applied away from the cell body, which was comparable with the value when the laser was focused at an infinite distance from the cells. We thus believed that the cell disappearance was due to normal cellular processes. However, for direct laser irradiation, focusing the laser partially on the gold coating along with the cell body might cause cell detachment. Conversely, a situation in which the laser beam had passed only through the cell body might not produce a heating effect; subsequently, the cell remained attached to the platform.