Special Section on Fluorescence Molecular Imaging Honoring Prof. Roger Tsien, a Pioneer in Biomedical Optics

Salivary protein markers: a noninvasive protein profile-based method for the early diagnosis of oral premalignancy and malignancy

[+] Author Affiliations
Ajeetkumar Patil, Khoobram S. Choudhari, Vayakkara Kolaprath Unnikrishnan, Santhosh Chidangil

Manipal University, Centre for Atomic and Molecular Physics, Manipal 576104, India

Nandita Shenoy, Ravikiran Ongole

Manipal College of Dental Sciences, Department of Oral Medicine and Radiology, Attavar, Mangalore 575001, India

Keerthilatha M. Pai

Manipal College of Dental Sciences, Department of Oral Medicine and Radiology, Manipal 576104, India

Vasudevan Bhaskaran Kartha

Udayagiri, Deonar, Sion Mumbai 400088

J. Biomed. Opt. 18(10), 101317 (Aug 19, 2013). doi:10.1117/1.JBO.18.10.101317
History: Received March 1, 2013; Revised July 3, 2013; Accepted July 3, 2013
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Abstract.  An ultra-sensitive hyphenated technique, high-performance liquid chromatography-laser-induced fluorescence detection protein profiling of saliva, is evaluated for early detection and diagnosis of oral premalignancy and malignancy. Calibration sets of protein profiles of unstimulated whole saliva are collected from clinically/pathologically normal, premalignant, and malignant subjects and used as standards. Three parameters—scores of factors, sum of squared residuals, and Mahalanobis distance—derived from principal component analysis of protein profiles of the standard calibration sets, and blind samples are used for “match/no-match” diagnosis of the blind samples. Analyses of the results show that the method is capable of differentiating normal, premalignant, and malignant conditions with the sensitivity and specificity of 79% and 78%, respectively. The technique provides a fast, highly objective (free from personal judgment and statistically defined), and noninvasive diagnostic method for screening and early detection of oral cancer.

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© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Ajeetkumar Patil ; Khoobram S. Choudhari ; Vayakkara Kolaprath Unnikrishnan ; Nandita Shenoy ; Ravikiran Ongole, et al.
"Salivary protein markers: a noninvasive protein profile-based method for the early diagnosis of oral premalignancy and malignancy", J. Biomed. Opt. 18(10), 101317 (Aug 19, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.10.101317


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