Research Papers: Imaging

Mitigating fluorescence spectral overlap in wide-field endoscopic imaging

[+] Author Affiliations
Chenying Yang

University of Washington, Department of Bioengineering, Seattle, Washington 98195

Vivian Hou

University of Washington, Department of Biology, Seattle, Washington 98195

Leonard Y. Nelson, Eric J. Seibel

University of Washington, Department of Mechanical Engineering, Seattle, Washington 98195

J. Biomed. Opt. 18(8), 086012 (Aug 21, 2013). doi:10.1117/1.JBO.18.8.086012
History: Received May 9, 2013; Revised July 9, 2013; Accepted July 23, 2013
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Abstract.  The number of molecular species suitable for multispectral fluorescence imaging is limited due to the overlap of the emission spectra of indicator fluorophores, e.g., dyes and nanoparticles. To remove fluorophore emission cross-talk in wide-field multispectral fluorescence molecular imaging, we evaluate three different solutions: (1) image stitching, (2) concurrent imaging with cross-talk ratio subtraction algorithm, and (3) frame-sequential imaging. A phantom with fluorophore emission cross-talk is fabricated, and a 1.2-mm ultrathin scanning fiber endoscope (SFE) is used to test and compare these approaches. Results show that fluorophore emission cross-talk could be successfully avoided or significantly reduced. Near term, the concurrent imaging method of wide-field multispectral fluorescence SFE is viable for early stage cancer detection and localization in vivo. Furthermore, a means to enhance exogenous fluorescence target-to-background ratio by the reduction of tissue autofluorescence background is demonstrated.

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© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Chenying Yang ; Vivian Hou ; Leonard Y. Nelson and Eric J. Seibel
"Mitigating fluorescence spectral overlap in wide-field endoscopic imaging", J. Biomed. Opt. 18(8), 086012 (Aug 21, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.8.086012


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