Research Papers: Imaging

Needle-based fluorescence endomicroscopy via structured illumination with a plastic, achromatic objective

[+] Author Affiliations
Matthew Kyrish, Jessica Dobbs, Rebecca Richards-Kortum, Tomasz S. Tkaczyk

Rice University, Department of Bioengineering, 6100 Main Street, Houston, Texas 77005

Shalini Jain, Xiao Wang, Dihua Yu

MD Anderson Cancer Center, Department of Molecular and Cellular Oncology, 1515 Holcombe Boulevard, Box 108, Houston, Texas 77030

J. Biomed. Opt. 18(9), 096003 (Sep 03, 2013). doi:10.1117/1.JBO.18.9.096003
History: Received May 30, 2013; Revised July 19, 2013; Accepted July 31, 2013
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Abstract.  In order to diagnose cancer, a sample must be removed, prepared, and examined under a microscope, which is expensive, invasive, and time consuming. Fiber optic fluorescence endomicroscopy, where an image guide is used to obtain high-resolution images of tissue in vivo, has shown promise as an alternative to conventional biopsies. However, the resolution of standard endomicroscopy is limited by the fiber bundle sampling frequency and out-of-focus light. A system is presented which incorporates a plastic, achromatic objective to increase the sampling and which provides optical sectioning via structured illumination to reject background light. An image is relayed from the sample by a fiber bundle with the custom 2.1-mm outer diameter objective lens integrated to the distal tip. The objective is corrected for the excitation and the emission wavelengths of proflavine (452 and 515 nm). It magnifies the object onto the fiber bundle to improve the system’s lateral resolution by increasing the sampling. The plastic lenses were fabricated via single-point diamond turning and assembled using a zero alignment technique. Ex vivo images of normal and neoplastic murine mammary tissues stained with proflavine are captured. The system achieves higher contrast and resolves smaller features than standard fluorescence endomicroscopy.

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© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Matthew Kyrish ; Jessica Dobbs ; Shalini Jain ; Xiao Wang ; Dihua Yu, et al.
"Needle-based fluorescence endomicroscopy via structured illumination with a plastic, achromatic objective", J. Biomed. Opt. 18(9), 096003 (Sep 03, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.9.096003


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