Research Papers: Imaging

Cross-correlation-based transverse flow measurements using optical resolution photoacoustic microscopy with a digital micromirror device

[+] Author Affiliations
Jinyang Liang, Yong Zhou, Konstantin I. Maslov, Lihong V. Wang

Washington University in St. Louis, Optical Imaging Laboratory, Department of Biomedical Engineering, 1 Brookings Drive, St. Louis, Missouri 63130

J. Biomed. Opt. 18(9), 096004 (Sep 03, 2013). doi:10.1117/1.JBO.18.9.096004
History: Received May 29, 2013; Revised August 7, 2013; Accepted August 9, 2013
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Abstract.  A cross-correlation-based method is proposed to quantitatively measure transverse flow velocity using optical resolution photoacoustic (PA) microscopy enhanced with a digital micromirror device (DMD). The DMD is used to alternately deliver two spatially separated laser beams to the target. Through cross-correlation between the slow-time PA profiles measured from the two beams, the speed and direction of transverse flow are simultaneously derived from the magnitude and sign of the time shift, respectively. Transverse flows in the range of 0.50 to 6.84mm/s are accurately measured using an aqueous suspension of 10-μm-diameter microspheres, and the root-mean-squared measurement accuracy is quantified to be 0.22mm/s. The flow measurements are independent of the particle size for flows in the velocity range of 0.55 to 6.49mm/s, which was demonstrated experimentally using three different sizes of microspheres (diameters: 3, 6, and 10 μm). The measured flow velocity follows an expected parabolic distribution along the depth direction perpendicular to the flow. Both maximum and minimum measurable velocities are investigated for varied distances between the two beams and varied total time for one measurement. This technique shows an accuracy of 0.35mm/s at 0.3-mm depth in scattering chicken breast, making it promising for measuring flow in biological tissue.

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© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Jinyang Liang ; Yong Zhou ; Konstantin I. Maslov and Lihong V. Wang
"Cross-correlation-based transverse flow measurements using optical resolution photoacoustic microscopy with a digital micromirror device", J. Biomed. Opt. 18(9), 096004 (Sep 03, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.9.096004


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