Research Papers: Sensing

Integrated optical microfluidic biosensor using a polycarbazole photodetector for point-of-care detection of hormonal compounds

[+] Author Affiliations
Nuno Miguel Matos Pires, Tao Dong, Ulrik Hanke, Nils Hoivik

Vestfold University College, Faculty of Technology and Maritime Sciences, Department of Micro and Nano Systems Technology, P.O. 2243, N-3103 Tonsberg, Norway

J. Biomed. Opt. 18(9), 097001 (Sep 03, 2013). doi:10.1117/1.JBO.18.9.097001
History: Received May 22, 2013; Revised July 30, 2013; Accepted August 13, 2013
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Abstract.  A picogram-sensitive optical microfluidic biosensor using an integrated polycarbazole photodiode is developed. The photodetector is mainly composed of the blend heterojunction of poly [N-9-heptadecanyl-2,7-carbazole-alt-5,5-(4,7-di-2-thienyl-2,1,3-benzothiadiazole)] (PCDTBT) and [6,6]-phenyl C71-butyric acid methyl ester (PC70BM) and the poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS) as the hole transport layer. Analyte detection is accomplished via a chemiluminescent immunoassay performed in a poly(dimethylsiloxane)-gold-glass hybrid microchip, on which antibodies were immobilized and chemiluminescent horseradish peroxidase-luminol-peroxide reactions were generated. Enhanced sensor response to the chemiluminescent light is achieved by optimizing the thickness of PCDTBT:PC70BM and PEDOT:PSS. Using the optimized polycarbazole photodiode for detecting the human thyroid-stimulating hormone as the model target, the integrated biosensor demonstrates an excellent linearity in the range of 0.03 to 10ng/ml with an analytical sensitivity of 68pg/ml. The sensor response shows high specificity and reproducibility. Hormone detection in clinical samples is further demonstrated and compared with a commercial enzyme-linked immunosorbent assay. The integrated device reported here has potential to detect other hormonal compounds or protein targets. Moreover, the presented concept enables the development of miniaturized, low-cost but highly sensitive optical microfluidic biosensors based on integrated polymer photodetectors with high potential for point-of-care diagnostics.

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© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Nuno Miguel Matos Pires ; Tao Dong ; Ulrik Hanke and Nils Hoivik
"Integrated optical microfluidic biosensor using a polycarbazole photodetector for point-of-care detection of hormonal compounds", J. Biomed. Opt. 18(9), 097001 (Sep 03, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.9.097001


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