Special Section on Advanced Biomedical Imaging and Sensing

Rapid detection of urinary polyomavirus BK by heterodyne-based surface plasmon resonance biosensor

[+] Author Affiliations
Li-Chen Su

Chang Gung University, Department of Electronic Engineering, Taoyuan 33002, Taiwan

Chang Gung University, Graduate Institute of Electro-Optical Engineering, Taoyuan 33302, Taiwan

Ya-Chung Tian

Chang Gung Memorial Hospital, Department of Nephrology, Kidney Research Center, Taoyuan 33305, Taiwan

Ying-Feng Chang

Chang Gung University, Graduate Institute of Electro-Optical Engineering, Taoyuan 33302, Taiwan

Chang Gung University, Molecular Medicine Research Center, Taoyuan 33302, Taiwan

Chien Chou

Chang Gung University, Graduate Institute of Electro-Optical Engineering, Taoyuan 33302, Taiwan

Chang Gung University, Biomedical Engineering Research Center, Taoyuan 33302, Taiwan

Chang Gung University, Healthy Aging Research Center, Taoyuan 33302, Taiwan

Chao-Sung Lai

Chang Gung University, Department of Electronic Engineering, Taoyuan 33002, Taiwan

Chang Gung University, Biomedical Engineering Research Center, Taoyuan 33302, Taiwan

J. Biomed. Opt. 19(1), 011013 (Sep 20, 2013). doi:10.1117/1.JBO.19.1.011013
History: Received April 3, 2013; Revised July 10, 2013; Accepted July 23, 2013
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Abstract.  In renal transplant patients, immunosuppressive therapy may result in the reactivation of polyomavirus BK (BKV), leading to polyomavirus-associated nephropathy (PVAN), which inevitably causes allograft failure. Since the treatment outcomes of PVAN remain unsatisfactory, early identification and continuous monitoring of BKV reactivation and reduction of immunosuppressants are essential to prevent PVAN development. The present study demonstrated that the developed dual-channel heterodyne-based surface plasmon resonance (SPR) biosensor is applicable for the rapid detection of urinary BKV. The use of a symmetrical reference channel integrated with the poly(ethylene glycol)-based low-fouling self-assembled monolayer to reduce the environmental variations and the nonspecific noise was proven to enhance the sensitivity in urinary BKV detection. Experimentally, the detection limit of the biosensor for BKV detection was estimated to be around 8500copies/mL. In addition, urine samples from five renal transplant patients were tested to rapidly distinguish PVAN-positive and PVAN-negative renal transplant patients. By virtue of its simplicity, rapidity, and applicability, the SPR biosensor is a remarkable potential to be used for continuous clinical monitoring of BKV reactivation.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Li-Chen Su ; Ya-Chung Tian ; Ying-Feng Chang ; Chien Chou and Chao-Sung Lai
"Rapid detection of urinary polyomavirus BK by heterodyne-based surface plasmon resonance biosensor", J. Biomed. Opt. 19(1), 011013 (Sep 20, 2013). ; http://dx.doi.org/10.1117/1.JBO.19.1.011013


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