Research Papers: Sensing

Infrared spectroscopic analysis of human and bovine articular cartilage proteoglycans using carbohydrate peak or its second derivative

[+] Author Affiliations
Lassi Rieppo

University of Eastern Finland, Department of Applied Physics, FI-70211 Kuopio, Finland

Kuopio University Hospital, Department of Clinical Neurophysiology, FI-70029 Kuopio, Finland

Tommi Närhi, Heikki J. Helminen

University of Eastern Finland, Institute of Biomedicine, FI-70211 Kuopio, Finland

Jukka S. Jurvelin

University of Eastern Finland, Department of Applied Physics, FI-70211 Kuopio, Finland

Simo Saarakkala

University of Oulu, Institute of Biomedicine, Department of Medical Technology, FI-90014 Oulu, Finland

Oulu University Hospital, Department of Diagnostic Radiology, FI-90029 Oulu, Finland

Jarno Rieppo

University of Eastern Finland, Institute of Biomedicine, FI-70211 Kuopio, Finland

Iisalmi Hospital, FI-74101 Iisalmi, Finland

J. Biomed. Opt. 18(9), 097006 (Sep 24, 2013). doi:10.1117/1.JBO.18.9.097006
History: Received July 3, 2013; Revised August 19, 2013; Accepted August 29, 2013
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Abstract.  Fourier transform infrared (FTIR) microspectroscopy has been used to estimate the spatial proteoglycan (PG) and collagen contents in articular cartilage (AC). However, it is not clear whether the results of FTIR analyses are consistent between different species. Our aim was to clarify how three different FTIR PG parameters in use, i.e., the integrated absorbance in the carbohydrate region, the carbohydrate/amide I ratio, and the second derivative peak at 1062cm1, can indicate the densitometrically assessed (reference method) spatial PG content in a sample set consisting of osteoarthritic human and bovine AC samples. The results show that all the parameters can accurately reflect the PG content, when the species are analyzed separately. When all samples are pooled, the correlation with the reference method is high (r=0.760, n=104) for the second derivative peak at 1062cm1 and is significantly lower (p<0.05) for the carbohydrate region (r=0.587, n=104) and for the carbohydrate/amide I ratio (r=0.579, n=104). Therefore, the analysis of the carbohydrate region may provide inconsistent results, if the cartilage samples from different species are in use. Based on the present study, second derivative analysis yields more consistent results for human and bovine cartilages.

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© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Lassi Rieppo ; Tommi Närhi ; Heikki J. Helminen ; Jukka S. Jurvelin ; Simo Saarakkala, et al.
"Infrared spectroscopic analysis of human and bovine articular cartilage proteoglycans using carbohydrate peak or its second derivative", J. Biomed. Opt. 18(9), 097006 (Sep 24, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.9.097006


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