Research Papers: Imaging

Investigation of transport dynamics in oleic acid–induced transdermal drug delivery by two-photon fluorescence microscopy: an ex-vivo study of mouse skin

[+] Author Affiliations
Te-Yu Tseng, Hsun-Chia Hsu, Chiu-Sheng Yang

National Taiwan University, Department of Physics, Room 530, Taipei 106, Taiwan

Tsung-Hua Tsai

Far Eastern Memorial Hospital, Department of Dermatology, New Taipei City 220, Taiwan

Chen-Yuan Dong

National Taiwan University, Department of Physics, Room 530, Taipei 106, Taiwan

National Taiwan University, Center for Quantum Science and Engineering, Taipei 106, Taiwan

National Taiwan University, Center for Optoelectronic Biomedicine, Taipei 106, Taiwan

J. Biomed. Opt. 18(9), 096016 (Sep 25, 2013). doi:10.1117/1.JBO.18.9.096016
History: Received January 31, 2013; Revised August 22, 2013; Accepted August 28, 2013
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Abstract.  Transdermal drug delivery, transporting the drug molecules through epidermis to dermis, has been extensively investigated but not studied in dynamic detail. The objective of this study is to monitor the dynamical changes of drug permeation and that of polarization of skin tissue with oleic acid treatment. We utilize two-photon fluorescence microscopy (TPM) to investigate the dynamics of transdermal drug delivery in skin excised from the abdominal region of euthenized nude mice with sulforhodamine B (SRB) modeling as a drug and Laurdan serving as a polarity indicator. The treatment of oleic acid increases the permeation rate of SRB, quickly reaching to the steady state of permeation. Increases in polarity within the skin tissue (in both intercellular and intracellular region of stratum corneum) are observed in SRB permeation enhanced by oleic acid treatment. TPM has successfully demonstrated the ability to study spatial distribution of transport dynamics in oleic acid-enhanced transdermal delivery.

Figures in this Article
© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Te-Yu Tseng ; Hsun-Chia Hsu ; Chiu-Sheng Yang ; Tsung-Hua Tsai and Chen-Yuan Dong
"Investigation of transport dynamics in oleic acid–induced transdermal drug delivery by two-photon fluorescence microscopy: an ex-vivo study of mouse skin", J. Biomed. Opt. 18(9), 096016 (Sep 25, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.9.096016


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