Special Section on Optical Imaging, Sensing, and Light Interactions in Cells and Tissues

Wide-field interferometric phase microscopy with molecular specificity using plasmonic nanoparticles

[+] Author Affiliations
Nir A. Turko, Anna Peled, Natan T. Shaked

Tel Aviv University, Department of Biomedical Engineering, Faculty of Engineering, Tel Aviv 69978, Israel

J. Biomed. Opt. 18(11), 111414 (Sep 27, 2013). doi:10.1117/1.JBO.18.11.111414
History: Received April 19, 2013; Revised July 26, 2013; Accepted July 31, 2013
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Abstract.  We present a method for adding molecular specificity to wide-field interferometric phase microscopy (IPM) by recording the phase signatures of gold nanoparticles (AuNPs) labeling targets of interest in biological cells. The AuNPs are excited by time-modulated light at a wavelength corresponding to their absorption spectral peak, evoking a photothermal (PT) effect due to their plasmonic resonance. This effect induces a local temperature rise, resulting in local refractive index and phase changes that can be detected optically. Using a wide-field interferometric phase microscope, we acquired an image sequence of the AuNP sample phase profile without requiring lateral scanning, and analyzed the time-dependent profile of the entire field of view using a Fourier analysis, creating a map of the locations of AuNPs in the sample. The system can image a wide-field PT phase signal from a cluster containing down to 16 isolated AuNPs. AuNPs are then conjugated to epidermal growth factor receptor (EGFR) antibodies and inserted to an EGFR-overexpressing cancer cell culture, which is imaged using IPM and verified by confocal microscopy. To the best of our knowledge, this is the first time wide-field interferometric PT imaging is performed at the subcellular level without the need for total internal reflection effects or scanning.

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© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Nir A. Turko ; Anna Peled and Natan T. Shaked
"Wide-field interferometric phase microscopy with molecular specificity using plasmonic nanoparticles", J. Biomed. Opt. 18(11), 111414 (Sep 27, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.11.111414


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