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Research Papers: Imaging

Photoacoustic recovery after photothermal bleaching in living cells

[+] Author Affiliations
Chiye Li, Chi Zhang, Liang Gao, Alejandro Garcia-Uribe, Lihong V. Wang

Washington University in St. Louis, Department of Biomedical Engineering, One Brookings Drive, St. Louis, Missouri 63130

J. Biomed. Opt. 18(10), 106004 (Oct 02, 2013). doi:10.1117/1.JBO.18.10.106004
History: Received April 22, 2013; Revised August 28, 2013; Accepted September 6, 2013
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Abstract.  We present an innovative method, photoacoustic recovery after photothermal bleaching (PRAP), for studying particle dynamics at micron scale via photoacoustic imaging. As an intuitive way to visualize and quantify dynamic processes, PRAP is demonstrated first in a simple phantom study and then in a more complex measurement involving live cells. Compared with the conventional fluorescence-based approach, PRAP provides high signal-to-noise ratio (SNR) imaging with minimal bleaching-induced artifacts during the recovery stage, ideal for monitoring the diffusive and kinetic processes inside a cell.

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© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Chiye Li ; Chi Zhang ; Liang Gao ; Alejandro Garcia-Uribe and Lihong V. Wang
"Photoacoustic recovery after photothermal bleaching in living cells", J. Biomed. Opt. 18(10), 106004 (Oct 02, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.10.106004


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