JBO Letters

Mesoscopic fluorescence molecular tomography of reporter genes in bioprinted thick tissue

[+] Author Affiliations
Mehmet S. Ozturk, Vivian K. Lee, Lingling Zhao, Guohao Dai, Xavier Intes

Rensselaer Polytechnic Institute, Biomedical Engineering Department, 110 8th Street, JEC#7036, Troy, New York 12180

J. Biomed. Opt. 18(10), 100501 (Oct 03, 2013). doi:10.1117/1.JBO.18.10.100501
History: Received July 18, 2013; Revised September 15, 2013; Accepted September 17, 2013
Text Size: A A A

Abstract.  Three-dimensional imaging of thick tissue constructs is one of the main challenges in the field of tissue engineering and regenerative medicine. Optical methods are the most promising as they offer noninvasive, fast, and inexpensive solutions. Herein, we report the use of mesoscopic fluorescence molecular tomography (MFMT) to image function and structure of thick bioprinted tissue hosted in a 3-mm-thick bioreactor. Collagen-based tissue assembled in this study contains two vascular channels formed by green fluorescent protein- and mCherry-expressing cells. Transfected live cell imaging enables us to image function, whereas Flash Red fluorescent bead perfusion into the vascular channel allows us to image structure. The MFMT optical reconstructions are benchmarked with classical microscopy techniques. MFMT and wide-field fluorescence microscopy data match within 92% in area and 84% in location, validating the accuracy of MFMT reconstructions. Our results demonstrate that MFMT is a well-suited imaging modality for fast, longitudinal, functional imaging of thick, and turbid tissue engineering constructs.

Figures in this Article
© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Mehmet S. Ozturk ; Vivian K. Lee ; Lingling Zhao ; Guohao Dai and Xavier Intes
"Mesoscopic fluorescence molecular tomography of reporter genes in bioprinted thick tissue", J. Biomed. Opt. 18(10), 100501 (Oct 03, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.10.100501


Tables

Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.