Research Papers: Imaging

High-resolution optoacoustic mesoscopy with a 24 MHz multidetector translate-rotate scanner

[+] Author Affiliations
Jérôme Gateau, Andrei Chekkoury, Vasilis Ntziachristos

Technische Universität München and Helmholtz Zentrum München, Institute for Biological and Medical Imaging, Ingoldstädter Landstraße 1, 85764 Neuherberg, Germany

J. Biomed. Opt. 18(10), 106005 (Oct 04, 2013). doi:10.1117/1.JBO.18.10.106005
History: Received June 26, 2013; Revised September 10, 2013; Accepted September 16, 2013
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Abstract.  Optoacoustic (photoacoustic) mesoscopy aims at high-resolution optical imaging of anatomical, functional, and cellular parameters at depths that go well beyond those of optical-resolution optical or optoacoustic microscopy i.e., reaching several millimeters in depth. The approach utilizes tomography to achieve ultrasonic-diffraction resolution and operates at high-ultrasound frequencies (20 to 200 MHz) induced by few-nanosecond laser pulse excitation of tissues. We investigated here the performance of optoacoustic mesoscopy implemented at 24 MHz center frequency and its ability to resolve optical absorption contrast in the mouse kidney ex vivo. The developed system achieved better than 30 μm in-plane resolution and 110 μm elevation resolution over a cylindrical volume of 9-mm diameter and 9-mm height. This unprecedented combination of resolution and depth was achieved by implementing a translate-rotate detection geometry and by tomographic reconstruction. The approach yielded images of optically absorbing structures with a level of detail never-before visualized in an intact mouse kidney and allows insights into their unperturbed architecture. We discuss the ability to offer multispectral acquisitions and enable in vivo imaging.

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© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Jérôme Gateau ; Andrei Chekkoury and Vasilis Ntziachristos
"High-resolution optoacoustic mesoscopy with a 24 MHz multidetector translate-rotate scanner", J. Biomed. Opt. 18(10), 106005 (Oct 04, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.10.106005


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