Research Papers: Therapeutic

CO2-lased enamel microhardness after brushing and cariogenic challenge

[+] Author Affiliations
Alessandra Marques Corrêa-Afonso, Cíntia Guimarães de Almeida, Maria Cristina Borsatto

Universidade de São Paulo, Departamento de Clínica Infantil, Faculdade de Odontologia de Ribeirão Preto, Ribeirão Preto, SP 14040-904, Brazil

Luciano Bachmann

Universidade de São Paulo, Departamento de Física, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Ribeirão Preto, SP 14040-901, Brazil

Regina Guenka Palma Dibb, Silmara Aparecida Milori Corona

Universidade de São Paulo, Departamento de Odontologia Restauradora, Faculdade de Odontologia de Ribeirão Preto, Ribeirão Preto, SP 14040-904, Brazil

J. Biomed. Opt. 18(10), 108003 (Oct 21, 2013). doi:10.1117/1.JBO.18.10.108003
History: Received July 24, 2013; Revised September 12, 2013; Accepted September 26, 2013
Text Size: A A A

Abstract.  This study aimed to assess how the wear that brushing promotes affects CO2 laser-irradiated enamel microhardness after cariogenic challenge in vitro. Forty fragments measuring 4×4mm were randomly assigned to four groups according to the enamel surface treatment: G1—control, G2—CO2-laser irradiation, G3—brushing, and G4—CO2 laser irradiation + brushing. A laser device emitting at 10.6 μm was used (power=0.5W, energy per pulse=0.05mJ, and frequency=10kHz). Specimens belonging to groups G3 and G4 were brushed (80,000 strokes) with a brushing simulator using toothpaste. Next, the samples were challenged with acid: the specimens were immersed in demineralizing and remineralizing solutions for 8 days. The acid resistance of enamel was evaluated by cross-sectional microhardness tests. The area under the curve (KHN×μm) was calculated. Analysis of variance (ANOVA) one-away and Fisher’s test were performed for the statistical analysis (p<0.05). Group G2 specimens (31,185±4706) were statistically different from specimens belonging to groups G1 (26,723±2446), G3 (28,194±1376), and G4 (28,207±2234), which were statistically similar. The brushing time used in the present study probably wore the CO2-lased enamel, so demineralization could not be prevented in the brushed group.

Figures in this Article
© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Alessandra Marques Corrêa-Afonso ; Luciano Bachmann ; Cíntia Guimarães de Almeida ; Regina Guenka Palma Dibb ; Silmara Aparecida Milori Corona, et al.
"CO2-lased enamel microhardness after brushing and cariogenic challenge", J. Biomed. Opt. 18(10), 108003 (Oct 21, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.10.108003


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