Research Papers: Imaging

Changes induced by peripheral nerve injury in the morphology and nanomechanics of sensory neurons

[+] Author Affiliations
Ouafa Benzina

Université Montpellier 2, Laboratoire Charles Coulomb UMR 5221, F-34095, Montpellier, France

Centre National de la Recherche Scientifique, Laboratoire Charles Coulomb UMR 5221, F-34095, Montpellier, France

Laboratoire LVBPPE- Centre de Biotechnologie de Sfax- BP «1177» Route de Sidi MANSOUR – Sfax 3018-Tunisie

Vivien Szabo, Mari-belle Saab, Thierry Cloitre, Csilla Gergely, Marta Martin

Université Montpellier 2, Laboratoire Charles Coulomb UMR 5221, F-34095, Montpellier, France

Centre National de la Recherche Scientifique, Laboratoire Charles Coulomb UMR 5221, F-34095, Montpellier, France

Olivier Lucas, Frédérique Scamps

INSERM U 1051 INM-Hôpital St Eloi, 80, rue Augustin Fliche, 34091 Montpellier Cedex 5, France

J. Biomed. Opt. 18(10), 106014 (Oct 28, 2013). doi:10.1117/1.JBO.18.10.106014
History: Received June 21, 2013; Revised September 5, 2013; Accepted September 19, 2013
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Abstract.  Peripheral nerve injury in vivo promotes a regenerative growth in vitro characterized by an improved neurite regrowth. Knowledge of the conditioning injury effects on both morphology and mechanical properties of live sensory neurons could be instrumental to understand the cellular and molecular mechanisms leading to this regenerative growth. In the present study, we use differential interference contrast microscopy, fluorescence microscopy, and atomic force microscopy (AFM) to show that conditioned axotomy, induced by sciatic nerve injury, does not increase somatic size of sensory neurons from adult mice lumbar dorsal root ganglia but promotes the appearance of longer and larger neurites and growth cones. AFM on live neurons is also employed to investigate changes in morphology and membrane mechanical properties of somas of conditioned neurons following sciatic nerve injury. Mechanical analysis of the soma allows distinguishing neurons having a regenerative growth from control ones, although they show similar shapes and sizes.

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© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Ouafa Benzina ; Vivien Szabo ; Olivier Lucas ; Mari-belle Saab ; Thierry Cloitre, et al.
"Changes induced by peripheral nerve injury in the morphology and nanomechanics of sensory neurons", J. Biomed. Opt. 18(10), 106014 (Oct 28, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.10.106014


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