JBO Letters

Fast depth-sensitive fluorescence measurements in turbid media using cone shell configuration

[+] Author Affiliations
Yi Hong Ong, Quan Liu

Nanyang Technological University, Division of Bioengineering, School of Chemical and Biomedical Engineering, Singapore 637457, Singapore

J. Biomed. Opt. 18(11), 110503 (Nov 18, 2013). doi:10.1117/1.JBO.18.11.110503
History: Received September 12, 2013; Revised October 22, 2013; Accepted October 23, 2013
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Abstract.  Depth-sensitive fluorescence spectroscopy in a two-layered epithelial tissue phantom was demonstrated using a cone shell configuration implemented by an axicon lens and a fiber assembly including five rings of collection fibers. Each collection fiber ring was located at a different radial distance away from the center, for which fluorescence measurements from all rings showed a larger range of sensitivities to the top and bottom layers compared to the previously reported cone shell configuration. Furthermore, multiple fluorescence spectra corresponding to a range of successive targeted depths can be obtained simultaneously, which shortened data acquisition dramatically and eliminated the mechanical moving components required for depth-sensitive optical measurements in the previous setup. This new setup therefore would be preferred in a clinical setting.

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© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Yi Hong Ong and Quan Liu
"Fast depth-sensitive fluorescence measurements in turbid media using cone shell configuration", J. Biomed. Opt. 18(11), 110503 (Nov 18, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.11.110503


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