Research Papers: Therapeutic

Method to detect the variants of the erythrocyte in a rat model of Aβ25–35-induced neurotoxicity based on micro-Raman spectroscopy

[+] Author Affiliations
Chen Di-ling, Li Ning, Zhang He-ming, Liu Song-hao

South China Normal University, Southern Institute of Pharmaceutical Research, Guangzhou 510631, China

Lin Li, Deng Shao-dong

Guangzhou University of Chinese Medicine, College of Chinese Materia Medical, Guangzhou 510006, China

J. Biomed. Opt. 18(11), 118003 (Nov 18, 2013). doi:10.1117/1.JBO.18.11.118003
History: Received August 29, 2013; Revised October 15, 2013; Accepted October 25, 2013
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Abstract.  Alzheimer’s disease irreversibly and progressively damages the brain, but the treatments in clinical trials are too slow. So, we hypothesized that the presence of erythrocyte variants with AD could be used as a noninvasive means to predict or trigger for administration of the preventive therapeutics, and the aim of this study is to develop a method using Raman spectroscopy in a rat model of Aβ2535-induced neurotoxicity, and then evaluate the protective effect of bajijiasu by this method. Results showed that the Raman spectra fingerprints of the erythrocyte of model group were obvious different from those of the normal control, as peaks around the region 650cm1 belonged to the s–s makers, 1605cm1 corresponded to the high spin (deoxygenated-Hb) marker, 1374cm1 arises from ν4 as a sign of concentration of O2, and 1123 and 1033cm1 are associated with the trans stretching vibrations of CAC skeleton. Results also showed that bajijiasu can make these changes recover. Our study also suggested that erythrocyte variants detected using Raman spectroscopy should be tested in a specific longitudinal study for the association with AD diagnosis, and if positive, can be used as a prognostic marker.

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© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Chen Di-ling ; Li Ning ; Lin Li ; Deng Shao-dong ; Zhang He-ming, et al.
"Method to detect the variants of the erythrocyte in a rat model of Aβ25–35-induced neurotoxicity based on micro-Raman spectroscopy", J. Biomed. Opt. 18(11), 118003 (Nov 18, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.11.118003


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