Research Papers: Sensing

Differentiation of cancer cells in two-dimensional and three-dimensional breast cancer models by Raman spectroscopy

[+] Author Affiliations
Nur P. Damayanti, Yi Fang, Joseph Irudayaraj

Purdue University, Department of Agricultural and Biological Engineering, 225 S. University Street, West Lafayette, Indiana 47907

Purdue University, Bindley Bioscience Center, West Lafayette, Indiana 47907

Mukti R. Parikh, Julia Kirshner

Purdue University, Department of Biological Sciences, 915 W. State Street, West Lafayette, Indiana 47907

Ana Paula Craig

Purdue University, Bindley Bioscience Center, West Lafayette, Indiana 47907

J. Biomed. Opt. 18(11), 117008 (Nov 18, 2013). doi:10.1117/1.JBO.18.11.117008
History: Received June 3, 2013; Revised October 10, 2013; Accepted October 15, 2013
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Abstract.  We demonstrate the first application of Raman spectroscopy in diagnosing nonmalignant, premalignant, malignant, and metastatic stages of breast cancer in a three-dimensional (3-D) cell culture model that closely mimics an in vivo environment. Comprehensive study comparing classification in two-dimensional (2-D) and 3-D cell models was performed using statistical methods composed of principal component analysis for exploratory analysis and outlier removal, partial least squares discriminant analysis, and elastic net regularized regression for classification. Our results show that Raman spectroscopy with an appropriate classification tool has excellent resolution to discriminate the four stages of breast cancer progression, with a near 100% accuracy for both 2-D and 3-D cell models. The diversity in chemical groups related to nucleic acids, proteins, and lipids, among other chemicals, were identified by appropriate peaks in the Raman spectra that correspond to the correct classification of the different stages of tumorigenesis model comprising of MCF10A, MCF10AneoT, MCF10CA1h, and MCF10CA1a cell lines. An explicit relationship between wavenumber and the stages of cancer progression was identified by the elastic net variable selection.

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© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Nur P. Damayanti ; Yi Fang ; Mukti R. Parikh ; Ana Paula Craig ; Julia Kirshner, et al.
"Differentiation of cancer cells in two-dimensional and three-dimensional breast cancer models by Raman spectroscopy", J. Biomed. Opt. 18(11), 117008 (Nov 18, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.11.117008


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