Special Section on Nanobio-Based Optical Sensing and Imaging

Localized surface plasmon resonance based nanobiosensor for biomarker detection of invasive cancer cells

[+] Author Affiliations
Yoochan Hong, Dae Sung Yoon

Yonsei University, Department of Biomedical Engineering, Maeji 234, Heungup, Wonju, Gangwondo 220-710, Republic of Korea

Minhee Ku

Yonsei University, College of Medicine, Department of Radiology, Shinchon, Seodaemoon, Seoul 120-752, Republic of Korea

Yonsei University, College of Medicine, Brain Korea 21 Project for Medical Science, Shinchon, Seodaemoon, Seoul 120-752, Republic of Korea

Eugene Lee

Yonsei University, College of Medicine, Department of Radiology, Shinchon, Seodaemoon, Seoul 120-752, Republic of Korea

Yonsei University, Nanomedical National Core Research Center, Shinchon, Seodaemoon, Seoul 120-752, Republic of Korea

Jin-Suck Suh, Yong-Min Huh

Yonsei University, College of Medicine, Department of Radiology, Shinchon, Seodaemoon, Seoul 120-752, Republic of Korea

YUHS-KRIBB Medical Convergence Research Institute, Shinchon, Seodaemoon, Seoul 120-749, Republic of Korea

Severance Biomedical Science Institute (SBSI), Shinchon, Seodaemoon, Seoul 120-752, Republic of Korea

Jaemoon Yang

Yonsei University, College of Medicine, Department of Radiology, Shinchon, Seodaemoon, Seoul 120-752, Republic of Korea

Severance Biomedical Science Institute (SBSI), Shinchon, Seodaemoon, Seoul 120-752, Republic of Korea

Yonsei University Health System, Severance Integrative Research Institute for Cerebral & Cardiovascular Diseases, Shinchon, Seodaemoon, Seoul 120-752, Republic of Korea

J. Biomed. Opt. 19(5), 051202 (Dec 02, 2013). doi:10.1117/1.JBO.19.5.051202
History: Received May 7, 2013; Revised July 12, 2013; Accepted August 20, 2013
Text Size: A A A

Abstract.  In this study, we describe the development of a cancer biomarker-sensitive nanobiosensor based on localized surface plasmon resonance that enables recognition for proteolytic activity of membrane type 1 matrix metalloproteinase (MT1-MMP) anchored on invasive cancer cells. First of all, we prepared biomarker-detectable substrate based on gold nanorods (GNRs) using nanoparticle adsorption method. The sensitivity of the sensing chip was confirmed using various solvents that have different refractive indexes. Subsequently, MT1-MMP–specific cleavable peptide was conjugated onto the surface of GNRs, and molecular sensing about proteolytic activity was conducted using MT1-MMP and cell lysates. Collectively, we developed a biomarker detectable sensor, which allows for the effective detection of proteolytic activity about MT1-MMP extracted from invasive cancer cells.

Figures in this Article
© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Yoochan Hong ; Minhee Ku ; Eugene Lee ; Jin-Suck Suh ; Yong-Min Huh, et al.
"Localized surface plasmon resonance based nanobiosensor for biomarker detection of invasive cancer cells", J. Biomed. Opt. 19(5), 051202 (Dec 02, 2013). ; http://dx.doi.org/10.1117/1.JBO.19.5.051202


Tables

Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.