Special Section on Nanobio-Based Optical Sensing and Imaging

Aptamer-conjugated gold nanorod for photothermal ablation of epidermal growth factor receptor-overexpressed epithelial cancer

[+] Author Affiliations
Jihye Choi, Eun Bi Choi, Hyun-Ouk Kim, Dong Joo Kim, Seungjoo Haam

Yonsei University, Department of Chemical and Biomolecular Engineering, Seoul 120-749, Republic of Korea

Yeonji Park, Jin-Suck Suh, Jaemoon Yang, Yong-Min Huh

Yonsei University, Department of Radiology, Seoul 120-752, Republic of Korea

Yoochan Hong

Yonsei University, Department of Biomedical Engineering, Wonju 220-710, Republic of Korea

Sung-Ho Ryu, Jung Hwan Lee

Pohang University of Science and Technology, POSTECH Aptamer Initiatives, Division of Integrative Bioscience and Biotechnology, Pohang, 790-784, Republic of Korea

J. Biomed. Opt. 19(5), 051203 (Dec 02, 2013). doi:10.1117/1.JBO.19.5.051203
History: Received May 2, 2013; Revised August 8, 2013; Accepted September 24, 2013
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Abstract.  Biomarker-specific photothermal nanoparticles that can efficiently sense markers that are overexpressed in distinguished adenocarcinomas have attracted much interest in an aspect of efficacy increase of cancer treatment. We demonstrated a promising prospect of a smart photothermal therapy agent employing anti-epidermal growth factor receptor aptamer (AptEGFR)-conjugated polyethylene glycol (PEG) layted gold nanorods (AptEGFR-PGNRs). The cetyltrimethylammonium bromide bilayer on GNRs was replaced with heterobifunctional PEG (COOH-PEG-SH) not only to serve as a biocompatible stabilizer and but also to conjugate AptEGFR. Subsequently, to direct photothermal therapy agent toward epithelial cancer cells, the carboxylated PEGylated GNRs (PGNRs) were further functionalized with AptEGFR using carbodiimide chemistry. Then, to assess the potential as biomarker-specific photothermal therapy agent of synthesized AptEGFR-PGNRs, the optical properties, biocompatibility, colloidal stability, binding affinity, and epicellial cancer cell killing efficacy in vitro/in vivo under near-infrared laser irradiation were investigated. As a result, AptEGFR-PGNRs exhibit excellent tumor targeting ability and feasibility of effective photothermal ablation cancer therapy.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Jihye Choi ; Yeonji Park ; Eun Bi Choi ; Hyun-Ouk Kim ; Dong Joo Kim, et al.
"Aptamer-conjugated gold nanorod for photothermal ablation of epidermal growth factor receptor-overexpressed epithelial cancer", J. Biomed. Opt. 19(5), 051203 (Dec 02, 2013). ; http://dx.doi.org/10.1117/1.JBO.19.5.051203


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