Research Papers: Therapeutic

Effect of 940 nm low-level laser therapy on osteogenesis in vitro

[+] Author Affiliations
Mohammed Mahmood Jawad

Universiti Sains Malaysia, School of Dental Sciences, Health Campus, Orthodontic Unit, Kota Bharu, Kelantan 16150, Malaysia

Alnahrain University, Baghdad, Iraq

Adam Husein

Universiti Sains Malaysia, School of Dental Sciences, Health Campus, Conservative Department, Kota Bharu, Kelantan 16150, Malaysia

Ahmad Azlina

Universiti Sains Malaysia, School of Dental Sciences, Health Campus, Kota Bharu, Kelantan 16150, Malaysia

Mohammad Khursheed Alam, Rozita Hassan

Universiti Sains Malaysia, School of Dental Sciences, Health Campus, Orthodontic Unit, Kota Bharu, Kelantan 16150, Malaysia

Rumaizi Shaari

Universiti Malaysia Kelantan, Clinical sciences, Faculty of Veterinary Medicine, Kota Bharu, Kelantan 16100, Malaysia

J. Biomed. Opt. 18(12), 128001 (Dec 10, 2013). doi:10.1117/1.JBO.18.12.128001
History: Received July 30, 2013; Revised November 1, 2013; Accepted November 5, 2013
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Abstract.  Bone regeneration is essential in medical treatment, such as in surgical bone healing and orthodontics. The aim of this study is to examine the effect of different powers of 940 nm diode low-level laser treatment (LLLT) on osteoblast cells during their proliferation and differentiation stages. A human fetal osteoblast cell line was cultured and treated with LLLT. The cells were divided into experimental groups according to the power delivered and periods of exposure per day for each laser power. The (3-(4,5-dimethylthiazol-2yl)-2,5 diphenyl tetrazolium bromide) (MTT) assay was used to determine cell proliferation. Both alkaline phosphatase and osteocalcin activity assays were assessed for cell differentiation. All treatment groups showed a significant increase in cell proliferation and differentiation compared to the control group. Regarding the exposure time, the subgroups treated with the LLLT for 6 min showed higher proliferation and differentiation rates for the powers delivered, the 300-mW LLLT group significantly increased the amount of cell proliferation. By contrast, the 100 and 200 mW groups showed significantly greater amounts of cell differentiation. These results suggest that the use of LLLT may play an important role in stimulating osteoblast cells for improved bone formation.

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© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Mohammed Mahmood Jawad ; Adam Husein ; Ahmad Azlina ; Mohammad Khursheed Alam ; Rozita Hassan, et al.
"Effect of 940 nm low-level laser therapy on osteogenesis in vitro", J. Biomed. Opt. 18(12), 128001 (Dec 10, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.12.128001


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