Research Papers: Therapeutic

Effects of phototherapy on cartilage structure and inflammatory markers in an experimental model of osteoarthritis

[+] Author Affiliations
Poliani Oliveira

Federal University of São Carlos, Department of Physiotherapy, Rod Washington Luis, Km 235, Monjolinho, São Carlos, São Paulo 13565-902, Brazil

Adventist University Center of São Paulo, Department of Physiotherapy`, Estrada de Itapecerica, 5859, São Paulo 05828-001, Brazil

Anderson Amaro Santos, Tamara Rodrigues, Carla Roberta Tim, Stela M. Mattiello, Nivaldo Antonio Parizotto

Federal University of São Carlos, Department of Physiotherapy, Rod Washington Luis, Km 235, Monjolinho, São Carlos, São Paulo 13565-902, Brazil

Karina Zambone Pinto, Fernanda Freitas Anibal

Federal University of São Paulo, Department of Morphology and Pathology, Rod Washington Luis, Km 235, Monjolinho, São Carlos, São Paulo 13565-902, Brazil

Angela Maria Paiva Magri, Kelly Rossetti Fernandes, Ana Claudia Muniz Rennó

Federal University of São Paulo, Department of Biosciences, Avenida Ana Costa, 95, Santos, São Paulo 11060-001, Brazil

J. Biomed. Opt. 18(12), 128004 (Dec 16, 2013). doi:10.1117/1.JBO.18.12.128004
History: Received May 27, 2013; Revised September 24, 2013; Accepted October 24, 2013
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Abstract.  The aim of this study was to evaluate the effects of laser phototherapy on the degenerative modifications on the articular cartilage after the anterior cruciate ligament transection (ACLT) in the knee of rats. Eighty male rats (Wistar) were distributed into four groups: intact control group (IG), injured control group (CG), injured laser treated group at 10J/cm2 (L10), and injured laser treated group at 50J/cm2 (L50). Animals were distributed into two subgroups, sacrificed in 5 and 8 weeks postsurgery. The ACLT was used to induce knee osteoarthritis in rats. After 2 weeks postsurgery, laser phototherapy initiated and it was performed for 15 and 30 sessions. The histological findings revealed that laser irradiation, especially at 10J/cm2, modulated the progression of the degenerative process, showing a better cartilage structure and lower number of condrocytes compared to the other groups. Laser phototherapy was not able to decrease the degenerative process measured by Mankin score and prevent the increase of cartilage thickness related to the degenerative process. Moreover, it did not have any effect in the biomodulation of the expression of markers IL1β, tumor necrosis factor-α, and metalloprotein-13. Furthermore, laser irradiated animals, at 50J/cm2 showed a lower amount of collagen type 1.

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© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Poliani Oliveira ; Anderson Amaro Santos ; Tamara Rodrigues ; Carla Roberta Tim ; Karina Zambone Pinto, et al.
"Effects of phototherapy on cartilage structure and inflammatory markers in an experimental model of osteoarthritis", J. Biomed. Opt. 18(12), 128004 (Dec 16, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.12.128004


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