Research Papers: Imaging

In vivo full-field en face correlation mapping optical coherence tomography

[+] Author Affiliations
Paul M. McNamara, Hrebesh M. Subhash

National University of Ireland, Tissue Optics and Microcirculation Imaging Facility, National Biophotonics and Imaging Platform, Galway, Ireland

Martin J. Leahy

National University of Ireland, Tissue Optics and Microcirculation Imaging Facility, National Biophotonics and Imaging Platform, Galway, Ireland

RCSI Royal College of Surgeons, 123 St. Stephens Green, Dublin 2, Ireland

J. Biomed. Opt. 18(12), 126008 (Dec 16, 2013). doi:10.1117/1.JBO.18.12.126008
History: Received August 26, 2013; Revised November 11, 2013; Accepted November 15, 2013
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Abstract.  A full-field optical coherence tomography (OCT) system has been developed for the purpose of performing nonscanning en face flow imaging. The light source is centered at 840 nm with a bandwidth of 50 nm resulting in an axial resolution of 8 μm in air. Microscope objectives with a numerical aperture of 0.1 were incorporated giving a transverse resolution of 5 μm. A magnification of 5.65 was measured, resulting in a field of view of 1260×945μm. Pairs of interference fringe images are captured with opposing phase and a two-step phase image reconstruction method is applied to reconstruct each en face image. The OCT frame rate is 10 Hz. A two-dimensional cross-correlation technique is applied to pairs of consecutive en face images in order to distinguish dynamic from static light-scatterers. The feasibility of the method was examined by simulating blood flow by creating a phantom with 5% intralipid solution. In vivo imaging of a Xenopus laevis tadpole was also performed in order to investigate the feasibility of imaging the vascular system. We present for what we believe to be the first time, the application of correlation mapping optical coherence tomography to full-field OCT to provide in vivo functional imaging of blood vessels.

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© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Paul M. McNamara ; Hrebesh M. Subhash and Martin J. Leahy
"In vivo full-field en face correlation mapping optical coherence tomography", J. Biomed. Opt. 18(12), 126008 (Dec 16, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.12.126008


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