Research Papers: Therapeutic

Investigation of low-level laser therapy potentiality on proliferation and differentiation of human osteoblast-like cells in the absence/presence of osteogenic factors

[+] Author Affiliations
Nora Bloise, Marco Vercellino

University of Pavia, Department of Molecular Medicine, Biochemistry Unit “A.Castellani,” INSTM UdR of Pavia, 27100 Pavia, Italy

University of Pavia, Interdipartimental Center for Tissue Engineering (CIT), 27100 Pavia, Italy

Gabriele Ceccarelli, Maria Gabriella Cusella De Angelis

University of Pavia, Department of Public Health, Experimental Medicine and Forensics, 27100 Pavia, Italy

University of Pavia, Interdipartimental Center for Tissue Engineering (CIT), 27100 Pavia, Italy

Paolo Minzioni

University of Pavia, Department of Electrical, Computer, and Biomedical Engineering, 27100 Pavia, Italy

Laura Benedetti

University of Pavia, Department of Public Health, Experimental Medicine and Forensics, 27100 Pavia, Italy

Marcello Imbriani

University of Pavia, Department of Public Health, Experimental Medicine and Forensics, 27100 Pavia, Italy

Department of Occupational Medicine, Ergonomics and Disability, Salvatore Maugeri Foundation, IRCCS, Laboratory of Nanotechnology, 27100 Pavia, Italy

Livia Visai

University of Pavia, Department of Molecular Medicine, Biochemistry Unit “A.Castellani,” INSTM UdR of Pavia, 27100 Pavia, Italy

Department of Occupational Medicine, Ergonomics and Disability, Salvatore Maugeri Foundation, IRCCS, Laboratory of Nanotechnology, 27100 Pavia, Italy

J. Biomed. Opt. 18(12), 128006 (Dec 23, 2013). doi:10.1117/1.JBO.18.12.128006
History: Received July 11, 2013; Revised November 15, 2013; Accepted November 20, 2013
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Abstract.  Several studies have shown that low-level laser irradiation (LLLI) has beneficial effects on bone regeneration. The objective of this study was to examine the in vitro effects of LLLI on proliferation and differentiation of a human osteoblast-like cell line (Saos-2 cell line). Cultured cells were exposed to different doses of LLLI with a semiconductor diode laser (659 nm; 10 mW power output). The effects of laser on proliferation were assessed daily up to seven days of culture in cells irradiated once or for three consecutive days with laser doses of 1 or 3J/cm2. The obtained results showed that laser stimulation enhances the proliferation potential of Saos-2 cells without changing their telomerase pattern or morphological characteristics. The effects on cell differentiation were assessed after three consecutive laser irradiation treatments in the presence or absence of osteo-inductive factors on day 14. Enhanced secretion of proteins specific for differentiation toward bone as well as calcium deposition and alkaline phosphatase activity were observed in irradiated cells cultured in a medium not supplemented with osteogenic factors. Taken together these findings indicate that laser treatment enhances the in vitro proliferation of Saos-2 cells, and also influences their osteogenic maturation, which suggest it is a helpful application for bone tissue regeneration.

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© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Nora Bloise ; Gabriele Ceccarelli ; Paolo Minzioni ; Marco Vercellino ; Laura Benedetti, et al.
"Investigation of low-level laser therapy potentiality on proliferation and differentiation of human osteoblast-like cells in the absence/presence of osteogenic factors", J. Biomed. Opt. 18(12), 128006 (Dec 23, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.12.128006


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