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Research Papers: Sensing

Mitochondrial respiratory complex I probed by delayed luminescence spectroscopy

[+] Author Affiliations
Irina Baran

“Carol Davila” University of Medicine and Pharmacy, Department of Biophysics, 8 Eroii Sanitari, 050474 Bucharest, Romania

Diana Ionescu

“Carol Davila” University of Medicine and Pharmacy, Department of Biophysics, 8 Eroii Sanitari, 050474 Bucharest, Romania

Simona Privitera

Istituto Nazionale di Fisica Nucleare—Laboratori Nazionali del Sud, 62 via S. Sofia, 95125 Catania, Italy

Agata Scordino

Istituto Nazionale di Fisica Nucleare—Laboratori Nazionali del Sud, 62 via S. Sofia, 95125 Catania, Italy

Università di Catania, Dipartimento di Fisica e Astronomia, 6 v.le Andrea Doria, I-95125 Catania, Italy

Maria Magdalena Mocanu

“Carol Davila” University of Medicine and Pharmacy, Department of Biophysics, 8 Eroii Sanitari, 050474 Bucharest, Romania

Francesco Musumeci

Istituto Nazionale di Fisica Nucleare—Laboratori Nazionali del Sud, 62 via S. Sofia, 95125 Catania, Italy

Università di Catania, Dipartimento di Fisica e Astronomia, 6 v.le Andrea Doria, I-95125 Catania, Italy

Rosaria Grasso

Istituto Nazionale di Fisica Nucleare—Laboratori Nazionali del Sud, 62 via S. Sofia, 95125 Catania, Italy

Marisa Gulino

Istituto Nazionale di Fisica Nucleare—Laboratori Nazionali del Sud, 62 via S. Sofia, 95125 Catania, Italy

Università degli Studi di Enna “Kore,” Facoltà di Ingegneria, Architettura e delle Scienze Motorie, Cittadella Universitaria, 94100 Enna, Italia

Adrian Iftime

“Carol Davila” University of Medicine and Pharmacy, Department of Biophysics, 8 Eroii Sanitari, 050474 Bucharest, Romania

Ioana Teodora Tofolean

“Carol Davila” University of Medicine and Pharmacy, Department of Biophysics, 8 Eroii Sanitari, 050474 Bucharest, Romania

Alexandru Garaiman

“Carol Davila” University of Medicine and Pharmacy, Department of Biophysics, 8 Eroii Sanitari, 050474 Bucharest, Romania

Alexandru Goicea

“Carol Davila” University of Medicine and Pharmacy, Department of Biophysics, 8 Eroii Sanitari, 050474 Bucharest, Romania

Ruxandra Irimia

“Carol Davila” University of Medicine and Pharmacy, Department of Biophysics, 8 Eroii Sanitari, 050474 Bucharest, Romania

Alexandru Dimancea

“Carol Davila” University of Medicine and Pharmacy, Department of Biophysics, 8 Eroii Sanitari, 050474 Bucharest, Romania

Constanta Ganea

“Carol Davila” University of Medicine and Pharmacy, Department of Biophysics, 8 Eroii Sanitari, 050474 Bucharest, Romania

J. Biomed. Opt. 18(12), 127006 (Dec 23, 2013). doi:10.1117/1.JBO.18.12.127006
History: Received September 1, 2013; Revised November 6, 2013; Accepted November 25, 2013
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Abstract.  The role of mitochondrial complex I in ultraweak photon-induced delayed photon emission [delayed luminescence (DL)] of human leukemia Jurkat T cells was probed by using complex I targeting agents like rotenone, menadione, and quercetin. Rotenone, a complex I-specific inhibitor, dose-dependently increased the mitochondrial level of reduced nicotinamide adenine dinucleotide (NADH), decreased clonogenic survival, and induced apoptosis. A strong correlation was found between the mitochondrial levels of NADH and oxidized flavin mononucleotide (FMNox) in rotenone-, menadione- and quercetin-treated cells. Rotenone enhanced DL dose-dependently, whereas quercetin and menadione inhibited DL as well as NADH or FMNox. Collectively, the data suggest that DL of Jurkat cells originates mainly from mitochondrial complex I, which functions predominantly as a dimer and less frequently as a tetramer. In individual monomers, both pairs of pyridine nucleotide (NADH/reduced nicotinamide adenine dinucleotide phosphate) sites and flavin (FMN-a/FMN-b) sites appear to bind cooperatively their specific ligands. Enhancement of delayed red-light emission by rotenone suggests that the mean time for one-electron reduction of ubiquinone or FMN-a by the terminal Fe/S center (N2) is 20 or 284 μs, respectively. All these findings suggest that DL spectroscopy could be used as a reliable, sensitive, and robust technique to probe electron flow within complex I in situ.

Figures in this Article
© 2013 Society of Photo-Optical Instrumentation Engineers

Citation

Irina Baran ; Diana Ionescu ; Simona Privitera ; Agata Scordino ; Maria Magdalena Mocanu, et al.
"Mitochondrial respiratory complex I probed by delayed luminescence spectroscopy", J. Biomed. Opt. 18(12), 127006 (Dec 23, 2013). ; http://dx.doi.org/10.1117/1.JBO.18.12.127006


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