A simple microfluidic device was fabricated directly on the CMOS imager. First, a design was developed in Autocad. Then, the design was cut out of double-sided tape (Adhesive Research, ARC are 92712, Glen Rock, Pennsylvania) using a laser cutter (Epilog 10000). A PDMS slab was made by mixing the base with the curing agent at a ratio (Sylgard 184). The mixture was poured in a cell culture dish and placed on the hot plate for a few minutes to remove bubbles and left at room temperature over night to cure. A small cube was cut after the PDMS had hardened and ports were punched using a 22 gauge blunt needle. One side of the tape was stuck to the PDMS aligned with the ports and the other side was stuck to the imager. Once the imager set up was ready, PTFE tubing of size 30 from Cole-Parmer, Vernon Hills, Illinois (Part No. EW-06417-11) was inserted into the ports. The input port was attached to a syringe (Easy touch insulin syringe U-100) loaded with the sample. The output port fed a microcentrifuge tube. During imaging, the sample from the insulin syringe was injected into the chamber by a syringe pump (New Era Pump Systems, Inc. NE-1002×, Framingdale, New York) at the rate of .