Research Papers: Imaging

Label-free photoacoustic microscopy of peripheral nerves

[+] Author Affiliations
Thomas Paul Matthews

Washington University in St. Louis, Department of Biomedical Engineering, Campus Box 1097, One Brookings Drive, St. Louis, Missouri 63130

Chi Zhang

Washington University in St. Louis, Department of Biomedical Engineering, Campus Box 1097, One Brookings Drive, St. Louis, Missouri 63130

Da-Kang Yao, Konstantin Maslov, Lihong V. Wang

Washington University in St. Louis, Department of Biomedical Engineering, Campus Box 1097, One Brookings Drive, St. Louis, Missouri 63130

J. Biomed. Opt. 19(1), 016004 (Jan 06, 2014). doi:10.1117/1.JBO.19.1.016004
History: Received September 17, 2013; Revised December 4, 2013; Accepted December 5, 2013
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Abstract.  Peripheral neuropathy is a common neurological problem that affects millions of people worldwide. Diagnosis and treatment of this condition are often hindered by the difficulties in making objective, noninvasive measurements of nerve fibers. Photoacoustic microscopy (PAM) has the ability to obtain high resolution, specific images of peripheral nerves without exogenous contrast. We demonstrated the first proof-of-concept imaging of peripheral nerves using PAM. As validated by both standard histology and photoacoustic spectroscopy, the origin of photoacoustic signals is myelin, the primary source of lipids in the nerves. An extracted sciatic nerve sandwiched between two layers of chicken tissue was imaged by PAM to mimic the in vivo case. Ordered fibrous structures inside the nerve, caused by the bundles of myelin-coated axons, could be observed clearly. With further technical improvements, PAM can potentially be applied to monitor and diagnose peripheral neuropathies.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Thomas Paul Matthews ; Chi Zhang ; Da-Kang Yao ; Konstantin Maslov and Lihong V. Wang
"Label-free photoacoustic microscopy of peripheral nerves", J. Biomed. Opt. 19(1), 016004 (Jan 06, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.1.016004


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