Special Section on Nanobio-Based Optical Sensing and Imaging

Simple method of DNA stretching on glass substrate for fluorescence imaging and spectroscopy

[+] Author Affiliations
Guru P. Neupane

IBS Center for Integrated Nanostructure Physics, Institute for Basic Science (IBS), Daejeon 305-701, Republic of Korea

Sungkyunkwan University, Department of Energy Science, Suwon 440-746, Republic of Korea

Krishna P. Dhakal

IBS Center for Integrated Nanostructure Physics, Institute for Basic Science (IBS), Daejeon 305-701, Republic of Korea

Sungkyunkwan University, Department of Energy Science, Suwon 440-746, Republic of Korea

Min Su Kim

IBS Center for Integrated Nanostructure Physics, Institute for Basic Science (IBS), Daejeon 305-701, Republic of Korea

Hyunsoo Lee

Wake Forest University, Department of Physics, Winston-Salem, North Carolina 27109

Martin Guthold

Wake Forest University, Department of Physics, Winston-Salem, North Carolina 27109

Vincent S. Joseph

Incheon National University, Department of Chemistry, Incheon 406-772, Republic of Korea

Jong-Dal Hong

Incheon National University, Department of Chemistry, Incheon 406-772, Republic of Korea

Jeongyong Kim

IBS Center for Integrated Nanostructure Physics, Institute for Basic Science (IBS), Daejeon 305-701, Republic of Korea

Sungkyunkwan University, Department of Energy Science, Suwon 440-746, Republic of Korea

Incheon National University, Department of Physics, Incheon 406-772, Republic of Korea

J. Biomed. Opt. 19(5), 051210 (Jan 09, 2014). doi:10.1117/1.JBO.19.5.051210
History: Received August 1, 2013; Revised December 4, 2013; Accepted December 13, 2013
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Abstract.  We demonstrate a simple method of stretching DNA to its full length, suitable for optical imaging and atomic force microscopy (AFM). Two competing forces on the DNA molecules, which are the electrostatic attraction between positively charged dye molecules (YOYO-1) intercalated into DNA and the negatively charged surface of glass substrate, and the centrifugal force of the rotating substrate, are mainly responsible for the effective stretching and the dispersion of single strands of DNA. The density of stretched DNA molecules could be controlled by the concentration of the dye-stained DNA solution. Stretching of single DNA molecules was confirmed by AFM imaging and the photoluminescence spectra of single DNA molecule stained with YOYO-1 were obtained, suggesting that our method is useful for spectroscopic analysis of DNA at the single molecule level.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Guru P. Neupane ; Krishna P. Dhakal ; Min Su Kim ; Hyunsoo Lee ; Martin Guthold, et al.
"Simple method of DNA stretching on glass substrate for fluorescence imaging and spectroscopy", J. Biomed. Opt. 19(5), 051210 (Jan 09, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.5.051210


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