Research Papers: Sensing

Multiplexed label-free optical biosensor for medical diagnostics

[+] Author Affiliations
Barbara Bottazzi

Humanitas Clinical and Research Center, Department of Inflammation and Immunology, 20089 Rozzano, Milan, Italy

Lucia Fornasari

University of Pavia, Physics Department, via Bassi 6, 27100 Pavia, Italy

Ana Frangolho

Plasmore S.r.l., Via Deledda 4, 21020 Ranco, Varese, Italy

Silvia Giudicatti

University of Pavia, Physics Department, via Bassi 6, 27100 Pavia, Italy

Alberto Mantovani

Humanitas Clinical and Research Center, Department of Inflammation and Immunology, 20089 Rozzano, Milan, Italy

Franco Marabelli

University of Pavia, Physics Department, via Bassi 6, 27100 Pavia, Italy

Gerardo Marchesini

Plasmore S.r.l., Via Deledda 4, 21020 Ranco, Varese, Italy

Paola Pellacani

Plasmore S.r.l., Via Deledda 4, 21020 Ranco, Varese, Italy

Rita Therisod

University of Pavia, Physics Department, via Bassi 6, 27100 Pavia, Italy

Andrea Valsesia

Plasmore S.r.l., Via Deledda 4, 21020 Ranco, Varese, Italy

J. Biomed. Opt. 19(1), 017006 (Jan 27, 2014). doi:10.1117/1.JBO.19.1.017006
History: Received September 19, 2013; Revised December 18, 2013; Accepted December 18, 2013
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Abstract.  This paper describes a new multiplexed label-free biosensor. The detection technology is based on nanostructured gold-polymer surfaces. These surfaces support surface plasmon resonance modes that can be probed by a miniaturized optical setup. The optical characterization of the sensing chip shows the sensitivity and the limit-of-detection to refractive index changes. Moreover, by studying the progressive adhesion of molecular monolayers of polyelectrolytes, the decay of the plasmonic mode electric field above the surface has been reconstructed. A multiplexed label-free biosensing device is then described and characterized in terms of sensitivity, lateral resolution, and sensitivity to a model biological assay. The sensitivity in imaging mode of the device is of the order of 106 refractive index units, while the measured lateral resolution is 6.25 μm within a field of view of several tenths of mm2, making the instrument unique in terms of multiplexing capability. Finally, the proof-of-concept application of the technology as a point-of-care diagnostic tool for an inflammatory marker is demonstrated.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Barbara Bottazzi ; Lucia Fornasari ; Ana Frangolho ; Silvia Giudicatti ; Alberto Mantovani, et al.
"Multiplexed label-free optical biosensor for medical diagnostics", J. Biomed. Opt. 19(1), 017006 (Jan 27, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.1.017006


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