Research Papers: General

Evaluation of composition and mineral structure of callus tissue in rat femoral fracture

[+] Author Affiliations
Mikael J. Turunen

University of Eastern Finland, Department of Applied Physics, POB 1627, FIN-70211 Kuopio, Finland

Sebastian Lages

Lund University, Division of Physical Chemistry, POB 124, SE-22100 Lund, Sweden

Ana Labrador

Lund University, MAX IV Laboratory, POB 118, SE-22100 Lund, Sweden

Ulf Olsson

Lund University, Division of Physical Chemistry, POB 124, SE-22100 Lund, Sweden

Magnus Tägil

Lund University, Department of Orthopaedics, Clinical Sciences, POB 118, SE-22100 Lund, Sweden

Jukka S. Jurvelin

University of Eastern Finland, Department of Applied Physics, POB 1627, FIN-70211 Kuopio, Finland

Hanna Isaksson

University of Eastern Finland, Department of Applied Physics, POB 1627, FIN-70211 Kuopio, Finland

Lund University, Department of Orthopaedics, Clinical Sciences, POB 118, SE-22100 Lund, Sweden

Lund University, Division of Solid Mechanics, POB 118, SE-22100 Lund, Sweden

J. Biomed. Opt. 19(2), 025003 (Feb 12, 2014). doi:10.1117/1.JBO.19.2.025003
History: Received September 15, 2013; Revised January 15, 2014; Accepted January 17, 2014
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Abstract.  Callus formation is a critical step for successful fracture healing. Little is known about the molecular composition and mineral structure of the newly formed tissue in the callus. The aim was to evaluate the feasibility of small angle x-ray scattering (SAXS) to assess mineral structure of callus and cortical bone and if it could provide complementary information with the compositional analyses from Fourier transform infrared (FTIR) microspectroscopy. Femurs of 12 male Sprague–Dawley rats at 9 weeks of age were fractured and fixed with an intramedullary 1.1 mm K-wire. Fractures were treated with the combinations of bone morphogenetic protein-7 and/or zoledronate. Rats were sacrificed after 6 weeks and both femurs were prepared for FTIR and SAXS analysis. Significant differences were found in the molecular composition and mineral structure between the fracture callus, fracture cortex, and control cortex. The degree of mineralization, collagen maturity, and degree of orientation of the mineral plates were lower in the callus tissue than in the cortices. The results indicate the feasibility of SAXS in the investigation of mineral structure of bone fracture callus and provide complementary information with the composition analyzed with FTIR. Moreover, this study contributes to the limited FTIR and SAXS data in the field.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Mikael J. Turunen ; Sebastian Lages ; Ana Labrador ; Ulf Olsson ; Magnus Tägil, et al.
"Evaluation of composition and mineral structure of callus tissue in rat femoral fracture", J. Biomed. Opt. 19(2), 025003 (Feb 12, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.2.025003


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