Research Papers: Imaging

Spectroscopic intravascular photoacoustic imaging of lipids in atherosclerosis

[+] Author Affiliations
Krista Jansen

Unit Experimental Cardiology, Thorax Center, Department of Biomedical Engineering, Erasmus MC, Rotterdam, PO Box 2040, 3000 CA Rotterdam, The Netherlands

Interuniversity Cardiology Institute of The Netherlands—Netherlands Heart Institute, PO Box 19258, 3501 DG, Utrecht, The Netherlands

Antonius FW van der Steen

Unit Experimental Cardiology, Thorax Center, Department of Biomedical Engineering, Erasmus MC, Rotterdam, PO Box 2040, 3000 CA Rotterdam, The Netherlands

Interuniversity Cardiology Institute of The Netherlands—Netherlands Heart Institute, PO Box 19258, 3501 DG, Utrecht, The Netherlands

Delft University of Technology, Department of Imaging Science and Technology, Lorentzweg 1, 2628 CJ Delft, The Netherlands

Min Wu, Heleen MM van Beusekom, Gijs van Soest

Unit Experimental Cardiology, Thorax Center, Department of Biomedical Engineering, Erasmus MC, Rotterdam, PO Box 2040, 3000 CA Rotterdam, The Netherlands

Geert Springeling

Experimental Medical Instruments, Department of Experimental Medical Instruments, Erasmus MC, PO Box 2040, 3000 CA Rotterdam, The Netherlands

Xiang Li, Qifa Zhou, K. Kirk Shung

University of Southern California, Department of Biomedical Engineering, Los Angeles, California 90089

Dominique PV de Kleijn

Interuniversity Cardiology Institute of The Netherlands—Netherlands Heart Institute, PO Box 19258, 3501 DG, Utrecht, The Netherlands

Cardiovascular Research Institute, National University Heart Center, 5 Lower Kent Ridge Road Singapore 119074, Singapore

University Medical Center Utrecht, Laboratory of Experimental Cardiology, Heidelberglaan 100 3584 CX Utrecht, The Netherlands

J. Biomed. Opt. 19(2), 026006 (Feb 12, 2014). doi:10.1117/1.JBO.19.2.026006
History: Received October 21, 2013; Revised January 13, 2014; Accepted January 13, 2014
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Abstract.  The natural history of atherosclerosis is marked by changes in the lipid biochemistry in the diseased arterial wall. As lesions become more vulnerable, different cholesterol species accumulate in the plaque. Understanding unstable atherosclerosis as a pharmacological and interventional therapeutic target requires chemically specific imaging of disease foci. In this study, we aim to image atherosclerotic plaque lipids and other vessel wall constituents with spectroscopic intravascular photoacoustics (sIVPA). sIVPA imaging can identify lipids in human coronary atherosclerotic plaque by relying on contrast in the near-infrared absorption spectra of the arterial wall components. Using reference spectra acquired on pure compounds, we analyzed sIVPA data from human coronary plaques ex vivo, to image plaque composition in terms of cholesterol and cholesterol ester content. In addition, we visualized the deeper lying connective tissue layers of the adventitia, as well as the fatty acid containing adipose cells in the peri-adventitial tissue. We performed simultaneous coregistered IVUS imaging to obtain complementary morphological information. Results were corroborated by histopathology. sIVPA imaging can distinguish the most prevalent lipid components of human atherosclerotic plaques and also visualize the connective tissue layers of the adventitia and the fatty acid containing adipose cells in the peri-adventitial tissue.

Figures in this Article
© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Krista Jansen ; Antonius FW van der Steen ; Min Wu ; Heleen MM van Beusekom ; Geert Springeling, et al.
"Spectroscopic intravascular photoacoustic imaging of lipids in atherosclerosis", J. Biomed. Opt. 19(2), 026006 (Feb 12, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.2.026006


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