Research Papers: Sensing

Calibration-free absolute quantification of particle concentration by statistical analyses of photoacoustic signals in vivo

[+] Author Affiliations
Yong Zhou

Washington University in St. Louis, Department of Biomedical Engineering, Optical Imaging Laboratory, One Brookings Drive, St. Louis, Missouri 63130

Junjie Yao

Washington University in St. Louis, Department of Biomedical Engineering, Optical Imaging Laboratory, One Brookings Drive, St. Louis, Missouri 63130

Konstantin I. Maslov

Washington University in St. Louis, Department of Biomedical Engineering, Optical Imaging Laboratory, One Brookings Drive, St. Louis, Missouri 63130

Lihong V. Wang

Washington University in St. Louis, Department of Biomedical Engineering, Optical Imaging Laboratory, One Brookings Drive, St. Louis, Missouri 63130

J. Biomed. Opt. 19(3), 037001 (Mar 03, 2014). doi:10.1117/1.JBO.19.3.037001
History: Received October 24, 2013; Revised January 27, 2014; Accepted January 28, 2014
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Abstract.  Currently, laser fluence calibration is typically required for quantitative measurement of particle concentration in photoacoustic imaging. Here, we present a calibration-free method to quantify the absolute particle concentration by statistically analyzing photoacoustic signals. The proposed method is based on the fact that Brownian motion induces particle count fluctuation in the detection volume. If the count of particles in the detection volume is assumed to follow the Poisson distribution, its expected value can be calculated by the photoacoustic signal mean and variance. We first derived a theoretical model for photoacoustic signals. Then, we applied our method to quantitative measurement of different concentrations of various particles, including red blood cells. Finally, we performed in vivo experiments to demonstrate the potential of our method in biological applications. The experimental results agreed well with the predictions from the theoretical model suggesting that our method can be used for noninvasive measurement of absolute particle concentrations in deep tissue without fluence calibration.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Yong Zhou ; Junjie Yao ; Konstantin I. Maslov and Lihong V. Wang
"Calibration-free absolute quantification of particle concentration by statistical analyses of photoacoustic signals in vivo", J. Biomed. Opt. 19(3), 037001 (Mar 03, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.3.037001


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