Research Papers: Imaging

Integrated photoacoustic, confocal, and two-photon microscope

[+] Author Affiliations
Bin Rao

Washington University in St. Louis, Optical Imaging Laboratory, Department of Biomedical Engineering, One Brookings Drive, St. Louis, Missouri 63130

Florentina Soto

Washington University School of Medicine, Department of Ophthalmology and Visual Sciences, Box 8096, St. Louis, Missouri 63110

Daniel Kerschensteiner

Washington University School of Medicine, Department of Ophthalmology and Visual Sciences, Box 8096, St. Louis, Missouri 63110

Lihong V. Wang

Washington University in St. Louis, Optical Imaging Laboratory, Department of Biomedical Engineering, One Brookings Drive, St. Louis, Missouri 63130

J. Biomed. Opt. 19(3), 036002 (Mar 03, 2014). doi:10.1117/1.JBO.19.3.036002
History: Received December 19, 2013; Revised January 27, 2014; Accepted January 28, 2014
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Abstract.  The invention of green fluorescent protein and other molecular fluorescent probes has promoted applications of confocal and two-photon fluorescence microscopy in biology and medicine. However, exogenous fluorescence contrast agents may affect cellular structure and function, and fluorescence microscopy cannot image nonfluorescent chromophores. We overcome this limitation by integrating optical-resolution photoacoustic microscopy into a modern Olympus IX81 confocal, two-photon, fluorescence microscope setup to provide complementary, label-free, optical absorption contrast. Automatically coregistered images can be generated from the same sample. Imaging applications in ophthalmology, developmental biology, and plant science are demonstrated. For the first time, in a familiar microscopic fluorescence imaging setting, this trimodality microscope provides a platform for future biological and medical discoveries.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Bin Rao ; Florentina Soto ; Daniel Kerschensteiner and Lihong V. Wang
"Integrated photoacoustic, confocal, and two-photon microscope", J. Biomed. Opt. 19(3), 036002 (Mar 03, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.3.036002


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