Research Papers: Imaging

Real-time label-free detection of dividing cells by means of lensfree video-microscopy

[+] Author Affiliations
Srikanth Vinjimore Kesavan, Fabrice P. Navarro, Mathilde Menneteau, Frederique Mittler, Jean-Marc Dinten, Cedric P. Allier

Commissariat à l’énergie atomique et aux énergies alternatives (CEA), LETI, MINATEC, 17 rue des martyrs, Grenoble cedex 9, 38054 France

Brigitte David-Watine, Nelly Dubrulle, Spencer L. Shorte

Plateforme d’imagerie dynamique, Imagopole, Institut Pasteur, Paris, 75015 France

Bernard Chalmond

University of Cergy-Pontoise, UFR Sciences, Cergy 95011 France

CMLA, École Normale Supérieure de Cachan, 94230 France

J. Biomed. Opt. 19(3), 036004 (Mar 05, 2014). doi:10.1117/1.JBO.19.3.036004
History: Received November 22, 2013; Revised January 8, 2014; Accepted January 23, 2014
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Abstract.  Quantification of cell proliferation and monitoring its kinetics are essential in fields of research such as developmental biology, oncology, etc. Although several proliferation assays exist, monitoring cell proliferation kinetics remains challenging. We present a novel cell proliferation assay based on real-time monitoring of cell culture inside a standard incubator using a lensfree video-microscope, combined with automated detection of single cell divisions over a population of several thousand cells. Since the method is based on direct visualization of dividing cells, it is label-free, continuous, and not sample destructive. Kinetics of cell proliferation can be monitored from a few hours to several days. We compare our method to a standard assay, the EdU proliferation assay, and as proof of principle, we demonstrate concentration-dependent and time-dependent effect of actinomycin D—a cell proliferation inhibitor.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Srikanth Vinjimore Kesavan ; Fabrice P. Navarro ; Mathilde Menneteau ; Frederique Mittler ; Brigitte David-Watine, et al.
"Real-time label-free detection of dividing cells by means of lensfree video-microscopy", J. Biomed. Opt. 19(3), 036004 (Mar 05, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.3.036004


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