As the interests in MG functions grow, several imaging modalities have been developed for visualization and investigation of the MGs and utilized for the diagnosis of the MG-related diseases [e.g., MG dysfunction (MGD)]. In a recent study by Ngo et al.,4 functionalities and characteristics of several MG visualization methods such as lid transillumination, video and noncontact meibography, confocal microscopy, ultrasound, and optical coherence tomography (OCT) were comprehensively provided. Owing to its capability of creating volumetric structure in a noninvasive way and with high contrast and resolution, OCT has been most recently utilized for visualizing the MGs.5,6 In the study,6 in order to investigate the morphological alternation of the MGs followed by different pathological conditions, in vivo three-dimensional (3-D)-OCT imaging was performed on the upper eyelid of subjects with normal MGs, MGD, and dry eye disease. For extracting a volumetric MG structure, the palpebral conjunctiva layer above the MGs was arbitrarily cropped out by the examiner from the scattering OCT intensity contrast. However, the accuracy of the differentiation between the conjunctiva layer and the MGs was not guaranteed because of the absence of any specific criteria for MG classification. Furthermore, due to the limited imaging range of , it seems to be difficult for observing the overall feature of the MG structure and for assessing the MG dropout (disappearance of the glandular tissue inside the tarsal plate). To our knowledge, for the first time, we demonstrate the functionality of polarization-sensitive OCT (PS-OCT) in terms of the visualization and investigation of MGs.