JBO Letters

Ultrasensitive opto-microfluidic immunosensor integrating gold nanoparticle–enhanced chemiluminescence and highly stable organic photodetector

[+] Author Affiliations
Nuno Miguel Matos Pires, Tao Dong

Buskerud and Vestfold University College, Department of Micro and Nano Systems Technology, Faculty of Technology and Maritime Sciences, P.O. 2243, N-3103 Tonsberg, Norway

J. Biomed. Opt. 19(3), 030504 (Mar 10, 2014). doi:10.1117/1.JBO.19.3.030504
History: Received December 31, 2013; Revised February 5, 2014; Accepted February 5, 2014
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Abstract.  The expensive fabrication of current opto-microfluidic sensors is a barrier to the successful adoption of these devices in point-of-care testing. This work reports a simple inexpensive opto-microfluidic device incorporating a poly(dimethylsiloxane)-glass hybrid microfluidic chip modified with gold nanoparticles and a high-detectivity, high-stability organic photodetector. The enhancing effect of the gold nanoparticles on horseradish peroxidase-luminol-H2O2 chemiluminescence was exploited in rapid single-analyte immunoassays. The limit of detection for 17-β estradiol was 2.5pg/ml, which is 200 times more sensitive than previously reported chemiluminescent immunosensors employing other organic photodetectors. Detection was also demonstrated in complex media, including natural water and blood serum.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Nuno Miguel Matos Pires and Tao Dong
"Ultrasensitive opto-microfluidic immunosensor integrating gold nanoparticle–enhanced chemiluminescence and highly stable organic photodetector", J. Biomed. Opt. 19(3), 030504 (Mar 10, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.3.030504


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