Special Section on Selected Topics in Biophotonics: Optical Coherence Tomography and Biomolecular Imaging with Coherent Raman Scattering Microscopy

Optical clearing at cellular level

[+] Author Affiliations
Matti Kinnunen

University of Oulu, Optoelectronics and Measurement Techniques Laboratory, P.O. Box 4500, Oulu 90014, Finland

Alexander V. Bykov

University of Oulu, Optoelectronics and Measurement Techniques Laboratory, P.O. Box 4500, Oulu 90014, Finland

Juho Tuorila

University of Oulu, Optoelectronics and Measurement Techniques Laboratory, P.O. Box 4500, Oulu 90014, Finland

Tomi Haapalainen

University of Oulu, Optoelectronics and Measurement Techniques Laboratory, P.O. Box 4500, Oulu 90014, Finland

Artashes V. Karmenyan

National Yang-Ming University, Biophotonics and Molecular Imaging Research Center, Taipei 11221, Taiwan

Valery V. Tuchin

University of Oulu, Optoelectronics and Measurement Techniques Laboratory, P.O. Box 4500, Oulu 90014, Finland

Saratov State University, Research-Educational Institute of Optics and Biophotonics, Saratov 410012, Russia

Institute of Precise Mechanics and Control RAS, Laboratory of Laser Diagnostics of Technical and Living Systems, Saratov 410028, Russia

J. Biomed. Opt. 19(7), 071409 (Mar 10, 2014). doi:10.1117/1.JBO.19.7.071409
History: Received November 20, 2013; Revised January 21, 2014; Accepted February 12, 2014
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Abstract.  Strong light scattering in tissues and blood reduces the usability of many optical techniques. By reducing scattering, optical clearing enables deeper light penetration and improves resolution in several optical imaging applications. We demonstrate the usage of optical tweezers and elastic light scattering to study optical clearing [one of the major mechanisms—matching of refractive indices (RIs)] at the single particle and cell level. We used polystyrene spheres and human red blood cells (RBCs) as samples and glycerol or glucose water solutions as clearing agents. Optical tweezers kept single microspheres and RBCs in place during the measurement of light scattering patterns. The results show that optical clearing reduces the scattering cross section and increases g. Glucose also decreased light scattering from a RBC. Optical clearing affected the anisotropy factor g of 23.25-μm polystyrene spheres, increasing it by 0.5% for an RI change of 2.2% (20% glycerol) and 0.3% for an RI change of 1.1% (13% glucose).

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Matti Kinnunen ; Alexander V. Bykov ; Juho Tuorila ; Tomi Haapalainen ; Artashes V. Karmenyan, et al.
"Optical clearing at cellular level", J. Biomed. Opt. 19(7), 071409 (Mar 10, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.7.071409


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