Research Papers: Imaging

R and G color component competition of RGB image decomposition as a criterion to register RBC agglutinates for blood group typing

[+] Author Affiliations
Valeri A. Doubrovski

Saratov State Medical University named after V.I. Razumovsky, Department of Medbiophysics, 112. Kazachaya Street, 410026 Saratov, Russia

Yuliya A. Ganilova

Saratov State Medical University named after V.I. Razumovsky, Department of Medbiophysics, 112. Kazachaya Street, 410026 Saratov, Russia

Igor V. Zabenkov

Saratov State Medical University named after V.I. Razumovsky, Department of Medbiophysics, 112. Kazachaya Street, 410026 Saratov, Russia

J. Biomed. Opt. 19(3), 036012 (Mar 17, 2014). doi:10.1117/1.JBO.19.3.036012
History: Received May 30, 2013; Revised January 27, 2014; Accepted January 28, 2014
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Abstract.  A new approach of the criterion assignment for registration of erythrocyte agglutinates to instrumentally determine blood group type is suggested. The criterion is based on comparison of R and G components of RGB decomposition of microscopy digital image taken for the blood-serum mixture sample. For the chosen experimental conditions, the minimal size (area) of RBC agglutinate to be registered by the criterion suggested is estimated theoretically. The proposed method was tested experimentally on the example of monitoring agglutinates in flow. The encouraging experimental results were obtained for improvement of the resolving power of the method; the optimal experimental conditions were revealed for maximum resolution. Though the suggested method was realized for dynamic (flow) blood group determination, it could also be applied for diagnostics in a stationary environment. This approach increases the reliability of RBC agglutinates registration and, hence, blood group typing. The results may be used to develop the apparatus for automated determination of human blood group.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Topics

Blood

Citation

Valeri A. Doubrovski ; Yuliya A. Ganilova and Igor V. Zabenkov
"R and G color component competition of RGB image decomposition as a criterion to register RBC agglutinates for blood group typing", J. Biomed. Opt. 19(3), 036012 (Mar 17, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.3.036012


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