Research Papers: Imaging

High-speed multispectral confocal biomedical imaging

[+] Author Affiliations
Gary E. Carver

Omega Optical Inc., 21 Omega Drive, Brattleboro, Vermont 05301

Sarah A. Locknar

Omega Optical Inc., 21 Omega Drive, Brattleboro, Vermont 05301

William A. Morrison

Omega Optical Inc., 21 Omega Drive, Brattleboro, Vermont 05301

V. Krishnan Ramanujan

Cedars-Sinai Medical Center, 8700 Beverly Boulevard, Los Angeles, California 90048

Daniel L. Farkas

University of Southern California, Department of Biomedical Engineering, Los Angeles, California 90089

Spectral Molecular Imaging, 250 N. Robertson Boulevard, Beverly Hills, California 90211

J. Biomed. Opt. 19(3), 036016 (Mar 21, 2014). doi:10.1117/1.JBO.19.3.036016
History: Received October 17, 2013; Revised January 20, 2014; Accepted February 12, 2014
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Abstract.  A new approach for generating high-speed multispectral confocal images has been developed. The central concept is that spectra can be acquired for each pixel in a confocal spatial scan by using a fast spectrometer based on optical fiber delay lines. This approach merges fast spectroscopy with standard spatial scanning to create datacubes in real time. The spectrometer is based on a serial array of reflecting spectral elements, delay lines between these elements, and a single element detector. The spatial, spectral, and temporal resolution of the instrument is described and illustrated by multispectral images of laser-induced autofluorescence in biological tissues.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Gary E. Carver ; Sarah A. Locknar ; William A. Morrison ; V. Krishnan Ramanujan and Daniel L. Farkas
"High-speed multispectral confocal biomedical imaging", J. Biomed. Opt. 19(3), 036016 (Mar 21, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.3.036016


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