Research Papers: General

Photobiological effect of low-level laser irradiation in bovine embryo production system

[+] Author Affiliations
Carlos Alexandre Soares

Universidade Federal do ABC, Center of Natural and Human Sciences, Rua Santa Adélia, 166, Bloco A, Torre 3, S642, Santo André, SP, Brazil

Kelly Annes

Universidade Federal do ABC, Center of Natural and Human Sciences, Rua Santa Adélia, 166, Bloco A, Torre 3, S642, Santo André, SP, Brazil

Thiago Revers Dreyer

Universidade Federal do ABC, Center of Natural and Human Sciences, Rua Santa Adélia, 166, Bloco A, Torre 3, S642, Santo André, SP, Brazil

Taciana Magrini

Universidade Federal do ABC, Center of Natural and Human Sciences, Rua Santa Adélia, 166, Bloco A, Torre 3, S642, Santo André, SP, Brazil

Mayra Trentin Sonoda

Universidade Federal do ABC, Center of Natural and Human Sciences, Rua Santa Adélia, 166, Bloco A, Torre 3, S642, Santo André, SP, Brazil

Herculano da Silva Martinho

Universidade Federal do ABC, Center of Natural and Human Sciences, Rua Santa Adélia, 166, Bloco A, Torre 3, S642, Santo André, SP, Brazil

Marcilio Nichi

University of São Paulo, Department of Animal Reproduction, São Paulo/ SP 05508-270, Brazil

Mayra Elena Ortiz d’Ávila Assumpção

University of São Paulo, Department of Animal Reproduction, São Paulo/ SP 05508-270, Brazil

Marcella Pecora Milazzotto

Universidade Federal do ABC, Center of Natural and Human Sciences, Rua Santa Adélia, 166, Bloco A, Torre 3, S642, Santo André, SP, Brazil

J. Biomed. Opt. 19(3), 035006 (Mar 21, 2014). doi:10.1117/1.JBO.19.3.035006
History: Received October 8, 2013; Revised January 23, 2014; Accepted February 19, 2014
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Abstract.  The objectives of this study were to evaluate the effect of low-level laser irradiation (LLLI) on bovine oocyte and granulosa cells metabolism during in vitro maturation (IVM) and further embryo development. Cumulus-oocytes complexes (COCs) were subjected (experimental group) or not (control group) to irradiation with LLLI in a 633-nm wavelength and 1J/cm2 fluency. The COCs were evaluated after 30 min, 8, 16, and 24 h of IVM. Cumulus cells were evaluated for cell cycle status, mitochondrial activity, and viability (flow cytometry). Oocytes were assessed for meiotic progression status (nuclear staining), cell cycle genes content [real-time polymerase chain reaction (PCR)], and signal transduction status (western blot). The COCs were also in vitro fertilized, and the cleavage and blastocyst rates were assessed. Comparisons among groups were statistically performed with 5% significance level. For cumulus cells, a significant increase in mitochondrial membrane potential and the number of cells progressing through the cycle could be observed. Significant increases on cyclin B and cyclin-dependent kinase (CDK4) levels were also observed. Concerning the oocytes, a significantly higher amount of total mitogen-activated protein kinase was found after 8 h of irradiation, followed by a decrease in all cell cycle genes transcripts, exception made for the CDK4. However, no differences were observed in meiotic progression or embryo production. In conclusion, LLLI is an efficient tool to modulate the granulosa cells and oocyte metabolism.

Figures in this Article
© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Carlos Alexandre Soares ; Kelly Annes ; Thiago Revers Dreyer ; Taciana Magrini ; Mayra Trentin Sonoda, et al.
"Photobiological effect of low-level laser irradiation in bovine embryo production system", J. Biomed. Opt. 19(3), 035006 (Mar 21, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.3.035006


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