Research Papers: Sensing

Tryptophan as the fingerprint for distinguishing aggressiveness among breast cancer cell lines using native fluorescence spectroscopy

[+] Author Affiliations
Lin Zhang

City College of the City University of New York, Department of Biomedical Engineering, 160 Convent Avenue, New York, New York 10031

Yang Pu

City College of the City University of New York, Department of Physics, Institute for Ultrafast Spectroscopy and Lasers, 160 Convent Avenue, New York, New York 10031

Jianpeng Xue

Washington University in St. Louis, Department of Radiology, School of Medicine, 4525 Scott Avenue, St. Louis, Missouri 63110

Sebastião Pratavieira

City College of the City University of New York, Department of Physics, Institute for Ultrafast Spectroscopy and Lasers, 160 Convent Avenue, New York, New York 10031

Universidade de São Paulo, Instituto de Física de São Carlos, São Carlos 13566-590, Brazil

Baogang Xu

Washington University in St. Louis, Department of Radiology, School of Medicine, 4525 Scott Avenue, St. Louis, Missouri 63110

Samuel Achilefu

Washington University in St. Louis, Department of Radiology, School of Medicine, 4525 Scott Avenue, St. Louis, Missouri 63110

Robert R. Alfano

City College of the City University of New York, Department of Physics, Institute for Ultrafast Spectroscopy and Lasers, 160 Convent Avenue, New York, New York 10031

J. Biomed. Opt. 19(3), 037005 (Mar 27, 2014). doi:10.1117/1.JBO.19.3.037005
History: Received August 30, 2013; Revised February 21, 2014; Accepted March 3, 2014
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Abstract.  Tryptophan is investigated as the key native marker in cells to determine the level of metastasis competence in breast cell lines using native fluorescence spectroscopy. The ratio of fluorescence intensity at 340 nm to intensity at 460 nm is associated with aggressiveness of the cancer cells. We found that the fluorescence of aggressive breast cancer cell has a much higher contribution from tryptophan compared with that from the normal cells and nonaggressive breast cancer cell.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Lin Zhang ; Yang Pu ; Jianpeng Xue ; Sebastião Pratavieira ; Baogang Xu, et al.
"Tryptophan as the fingerprint for distinguishing aggressiveness among breast cancer cell lines using native fluorescence spectroscopy", J. Biomed. Opt. 19(3), 037005 (Mar 27, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.3.037005


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